Bibliographic citations
Cordero, A., Chin, K., Tarazona, L. (2024). Evaluación de la citotoxicidad de la Persea caerulea (Ruiz & Pav.) Mez, y su actividad antiinflamatoria frente a artritis reumatoide inducida en ratas Lewis [Universidad Peruana Cayetano Heredia]. https://hdl.handle.net/20.500.12866/15183
Cordero, A., Chin, K., Tarazona, L. Evaluación de la citotoxicidad de la Persea caerulea (Ruiz & Pav.) Mez, y su actividad antiinflamatoria frente a artritis reumatoide inducida en ratas Lewis []. PE: Universidad Peruana Cayetano Heredia; 2024. https://hdl.handle.net/20.500.12866/15183
@misc{renati/911209,
title = "Evaluación de la citotoxicidad de la Persea caerulea (Ruiz & Pav.) Mez, y su actividad antiinflamatoria frente a artritis reumatoide inducida en ratas Lewis",
author = "Tarazona Perez, Linda Mayra",
publisher = "Universidad Peruana Cayetano Heredia",
year = "2024"
}
Background: Rheumatoid arthritis (RA) is an autoimmune disease that causes joint damage and disability. Persea caerulea, a plant used in traditional Peruvian medicine, may have anti-inflammatory effects due to its flavonoids. Objectives: To evaluate the cytotoxicity of the hydroalcoholic extract of P. caerulea and its anti-inflammatory activity in Lewis rats with RA. Materials and Methods: The cytotoxicity of P. caerulea was determined on 3T3, Vero, DU145, and SH-SY5Y cells by its IC50. Thirty Lewis rats were divided into six groups, five with induced RA and three controls. Three groups received P. caerulea (250, 500, and 1000 mg/kg) for 42 days. They were evaluated with inflammation scores and leg thickness. Histology and TNF-alpha levels were examined post-mortem. Results: The average IC50 values were 60, 130, 194, and 20 μg/mL in 3T3, VERO, DU145, and SH-SY5Y, respectively. Groups treated with 500 and 1000 mg/kg of P. caerulea showed increased joint thickness (p<0.05), with no significant changes in the inflammation score. There was more synovial inflammation in rats with 250 and 500 mg/kg of P. caerulea and more bone erosion in rats with 500 and 1000 mg/kg (p<0.05) compared to the placebo group. Conclusion: The extract at ≤ 250 mg/mL was not cytotoxic to the cell lines. It showed anti-inflammatory effects in the first week but aggravated the disease thereafter. Further studies are needed to evaluate its short-term anti-inflammatory effect.
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