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Nacarino, D., (2023). Estudio de la función de HRP3 en la síntesis de hemozoína en Plasmodium falciparum [Universidad Peruana Cayetano Heredia]. https://hdl.handle.net/20.500.12866/15211
Nacarino, D., Estudio de la función de HRP3 en la síntesis de hemozoína en Plasmodium falciparum []. PE: Universidad Peruana Cayetano Heredia; 2023. https://hdl.handle.net/20.500.12866/15211
@misc{renati/911204,
title = "Estudio de la función de HRP3 en la síntesis de hemozoína en Plasmodium falciparum",
author = "Nacarino Chavez, Daniela",
publisher = "Universidad Peruana Cayetano Heredia",
year = "2023"
}
Title: Estudio de la función de HRP3 en la síntesis de hemozoína en Plasmodium falciparum
Authors(s): Nacarino Chavez, Daniela
Keywords: P. falciparum; HRP2; HRP3; Proteína Rica en Histidina; Hemozoína; Hemoglobina
OCDE field: https://purl.org/pe-repo/ocde/ford#3.02.06; https://purl.org/pe-repo/ocde/ford#3.03.06; https://purl.org/pe-repo/ocde/ford#3.03.07; https://purl.org/pe-repo/ocde/ford#3.03.08
Issue Date: 2023
Institution: Universidad Peruana Cayetano Heredia
Abstract: A nivel global se han registrado 247 millones de casos de malaria en 84 países endémicos, siendo África el continente con una mayor prevalencia. Esta enfermedad infecciosa es causada por 5 especies de parásitos del género Plasmodium y se transmite vía mosquitos Anopheles spp. El responsable del paludismo severo es Plasmodium falciparum, el cual es la segunda especie más prevalente en la región amazónica del Perú. Durante su ciclo de vida intraeritrocitario, ocurre el mecanismo metabólico del grupo hemo. Este cofactor es crucial para su desarrollo y principalmente derivado de la digestión de la hemoglobina. Cuando se encuentra en altas concentraciones, es regulado por reacciones antioxidantes con proteínas, como la proteína rica en histidina 2 (HRP2), para convertir las fracciones hemo tóxicas a hemozoína a través de una biocristalización. La HRP2 se usa en el diagnóstico, pero no se sabe de manera completa su rol biológico, importancia, ni tampoco se han realizado estudios enfocados en su proteína homóloga HRP3. La investigación sobre la HRP2 y HRP3 en el metabolismo de la hemoglobina es muy limitada. Un estudio registró que la interrupción del gen pfhrp2 altera esta vía metabólica durante los estadios intraeritrocitarios, de manera que los niveles de transcripción de las enzimas involucradas fueron reducidos. Sin embargo, no se ha verificado de manera conjunta con el gen pfhrp3, ni se ha asociado con el fitness del parásito. El presente proyecto busca determinar si la HRP3 tiene la misma función que HRP2 de sintetizar la hemozoína durante el metabolismo de la hemoglobina, y si la disminución de esta síntesis podría estar relacionada a la reducción del fitness cuando hay doble deleción de los genes pfhrp2 y pfhrp3 de P. falciparum, reportado en un previo estudio.
Globally, 247 million cases of m alaria have been r ecorded in 84 endemic countries , with Africa being the continent with the highest prevalence. This infectious disease is caused by five species of Plasmodium parasites and is transmitted via mosquitoes Anopheles spp. The principal responsible for severe malaria is Plasmodium falciparum , w hich is the second most prevalent in the Peruvian Amazon. During its intraerythrocytic life cycle, the metabolic mechanism of the heme group occurs. This c ofactor is crucial for the development of the parasite and mainly derived from digestion of hemoglobin. When it is present in high concentrations , it is regulated by antioxidant reactions with proteins, such as histidine rich protein 2 (HRP2), in order to convert toxic heme fractions to hemozoin through biocrystallization. HRP2 is usually used in diagnostics, but its biological role and importance are not f ully studied, nor have studies focused on its homo logous protein HRP3. The research on HRP2 a nd HRP3 involved in hemoglobin metabolism is very limited. A recent study reported that disruption of the pfhrp2 gene alters this pathway during intraerythrocytic stages, so that transcript levels of the enzymes involved were reduced. Despite this, it has not been verified with the pfhrp3 gene, nor has it been associated with the parasite fitness. The proposal of the present project is to determine whether HRP3 has the same function as HRP2 in the hemozoin synthesis during hemoglobin metabolism, and whether the decrease in this synthesis could be related to reduced fitness when there is a double deletion of the P. falciparum pfhrp 2 and pfhrp3 genes, as reported in a previous study.
Globally, 247 million cases of m alaria have been r ecorded in 84 endemic countries , with Africa being the continent with the highest prevalence. This infectious disease is caused by five species of Plasmodium parasites and is transmitted via mosquitoes Anopheles spp. The principal responsible for severe malaria is Plasmodium falciparum , w hich is the second most prevalent in the Peruvian Amazon. During its intraerythrocytic life cycle, the metabolic mechanism of the heme group occurs. This c ofactor is crucial for the development of the parasite and mainly derived from digestion of hemoglobin. When it is present in high concentrations , it is regulated by antioxidant reactions with proteins, such as histidine rich protein 2 (HRP2), in order to convert toxic heme fractions to hemozoin through biocrystallization. HRP2 is usually used in diagnostics, but its biological role and importance are not f ully studied, nor have studies focused on its homo logous protein HRP3. The research on HRP2 a nd HRP3 involved in hemoglobin metabolism is very limited. A recent study reported that disruption of the pfhrp2 gene alters this pathway during intraerythrocytic stages, so that transcript levels of the enzymes involved were reduced. Despite this, it has not been verified with the pfhrp3 gene, nor has it been associated with the parasite fitness. The proposal of the present project is to determine whether HRP3 has the same function as HRP2 in the hemozoin synthesis during hemoglobin metabolism, and whether the decrease in this synthesis could be related to reduced fitness when there is a double deletion of the P. falciparum pfhrp 2 and pfhrp3 genes, as reported in a previous study.
Link to repository: https://hdl.handle.net/20.500.12866/15211
Discipline: Biología
Grade or title grantor: Universidad Peruana Cayetano Heredia. Facultad de Ciencias e Ingeniería Alberto Cazorla Talleri
Grade or title: Bachiller en Ciencias con mención en Biología
Juror: Del Aguila Soto, Segundo Wilman; Gutiérrez Loli, Renzo Marcelo
Register date: 21-Mar-2024
This item is licensed under a Creative Commons License