Bibliographic citations
Arroyo, G., (2022). Uso combinado de los patrones de bandas del ensayo de electro inmunotransferencia blot (EITB) y el ensayo de ELISA de captura de antígeno para el diagnóstico de infección viable en neurocisticercosis parenquimal humana y cisticercosis porcina [Universidad Peruana Cayetano Heredia]. https://hdl.handle.net/20.500.12866/13106
Arroyo, G., Uso combinado de los patrones de bandas del ensayo de electro inmunotransferencia blot (EITB) y el ensayo de ELISA de captura de antígeno para el diagnóstico de infección viable en neurocisticercosis parenquimal humana y cisticercosis porcina []. PE: Universidad Peruana Cayetano Heredia; 2022. https://hdl.handle.net/20.500.12866/13106
@phdthesis{renati/910582,
title = "Uso combinado de los patrones de bandas del ensayo de electro inmunotransferencia blot (EITB) y el ensayo de ELISA de captura de antígeno para el diagnóstico de infección viable en neurocisticercosis parenquimal humana y cisticercosis porcina",
author = "Arroyo Hurtado, Gianfranco",
publisher = "Universidad Peruana Cayetano Heredia",
year = "2022"
}
Background: The diagnosis of viable infection in human neurocysticercosis (NCC) allows the appropriate therapeutic approach to the patient and in porcine cysticercosis can provide information on the presence of Taenia solium in the environment to establish future control programs. In both cases the diagnosis of viable infection is complemented by serology. The detection of one or more antibody bands in the Electro Immunotransfer Blot (EITB) assay is highly sensitive (~100%), but poorly specific for viable infection, although EITB banding patterns do not occur randomly, their distribution reflects the immunity profile against 3 T. solium antigenic families (GP50, T24/42 and 8kDa) and can provide additional information on viability. On the other hand, serum antigen detection allows to discriminate viable infection, although its sensitivity in human NCC with single viable infections is low and its specificity in porcine cysticercosis is affected by cross-reaction with T. hydatigena. However, the combination of EITB banding patterns and antigen detection would allow a more appropriate discrimination of viable cysticercosis in both infections. Objective/Methods: We evaluated the combined ability of EITB banding patterns and Ag-ELISA results to discriminate viable infection using neuroimaging findings (magnetic resonance [MR]/computed tomography [CT]) in patients with parenchymal NCC (study 1, n = 214) and necropsy results in field pigs. (study 2, n = 469). In both studies, EITB banding patterns were grouped into homogeneous classes by latent class analysis and the proportion of cases with or without viable infection according to their Ag-ELISA results (positive/negative) in each latent class was assessed using Fisher's exact test. Results: In both studies, EITB banding patterns were grouped into four classes: 1 (EITB-negative or only positive to GP50 [GP50 antigenic family]), 2 (positive to GP42-39 and/or GP24 [antigenic family T24/42] with or without GP50), 3 and 4 (positive to GP50, GP42-39, GP24, and also positive to 8-kDa antigens [GP21, GP18, GP14 and GP13]). In study 1, the combination of EITB banding patterns and Ag-ELISA results allowed us to identify or rule out groups of patients with viable NCC with a high probability, mainly in class 2 (100% of Ag-ELISA positive cases had viable NCC, while 27.5% of Ag-ELISA negative cases had viable NCC, P < 0.001), to a lesser extent in classes 3 and 1, but not in class 4. In study 2, negative Ag-ELISA results, alone or in combination with EITB responses of classes 1, 2, and 3 allowed to ruled out viable porcine cysticercosis with a high probability (≥97%), although the ability of positive Ag-ELISA results to detect viable infection in EITB classes 1, 2 and 3 was only low or moderate (3.7%, 27.1%, and 67.9%) and did not contribute in class 4 (all pigs in this class had viable infection). Conclusions: The combination of EITB banding patterns (mainly class 2) and Ag- ELISA results in hospital settings can more appropriately complement neuroimaging to discriminate viable NCC and would also allow prioritizing imaging screening for viable NCC in areas with poor access to neuroimaging. In porcine populations, the combination of negative Ag-ELISA results with EITB banding patterns (classes 1 and 2 )may help to rule out viable infection and could be useful for evaluating prophylactic or therapeutic interventions, but positive Ag- ELISA results with EITB banding patterns do not adequately discriminate viable infection and should be interpreted with caution.
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