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Bocanegra, L., Izuiza, K. (2024). Efecto del extracto acetónico de las hojas de Momordica charantia L. sobre Cutibacterium acnes [Universidad Nacional de Trujillo]. https://hdl.handle.net/20.500.14414/22476
Bocanegra, L., Izuiza, K. Efecto del extracto acetónico de las hojas de Momordica charantia L. sobre Cutibacterium acnes []. PE: Universidad Nacional de Trujillo; 2024. https://hdl.handle.net/20.500.14414/22476
@misc{renati/883431,
title = "Efecto del extracto acetónico de las hojas de Momordica charantia L. sobre Cutibacterium acnes",
author = "Izuiza Polo, Kaneny Rene",
publisher = "Universidad Nacional de Trujillo",
year = "2024"
}
Title: Efecto del extracto acetónico de las hojas de Momordica charantia L. sobre Cutibacterium acnes
Advisor(s): Silva Correa, Carmen Rosa; Sagástegui Guarniz, William Antonio
OCDE field: https://purl.org/pe-repo/ocde/ford#3.01.05
Issue Date: 2024
Institution: Universidad Nacional de Trujillo
Abstract: El presente estudio tuvo como objetivo evaluar el efecto del extracto acetónico de las hojas de Momordica charantia L. sobre Cutibacterium acnes. Para tal fin el extracto acetónico previamente preparado se solubilizó en dimetilsulfóxido al 1% a una concentración de 2000 g/mL y se realizaron diluciones hasta llegar a la concentración de 3,91μg/mL. La cepa Cutibacterium acnes ATCC 6919 se sembró en placas de agar tríptica de soja (TSA) suplementadas con sangre de oveja al 5%. Se incubó a 37 °C y luego se transfirió a caldo cerebro corazón (BHI) suplementado con dextrosa al 1%. Mediante la escala de McFarland 0,5 se estandarizaron los inóculos bacterianos y se añadió 100 μL de caldo de cultivo (20 μL de extracto y 80 μL de inóculo). Doxiciclina fue el control positivo en concentraciones de 8 μg/mL a 0,015 μg/mL. Las microplacas se incubaron por 24 h a 37°C y se agregó 10 μL de resazurina (0,01%) dejando incubar a 37° C durante 2 h. La coloración azul se interpretó como ausencia de crecimiento bacteriano y la coloración rosa como crecimiento. Se determinó la concentración mínima inhibitoria (CMI) que fue de 500 ug/mL y mediante el UPLC-MS se halló como componentes mayoritarios Mormordicina IV y Kuguacina S.
ABSTRACT The present study aimed to evaluate the effect of the acetone extract of the leaves of Momordica charantia L. on Cutibacterium acnes. For this purpose, the previously prepared acetone extract was solubilized in 1% dimethylsulfoxide at a concentration of 2000 µg/mL and and diluted to a concentration of 3.91μg/mL. The strain Cutibacterium acnes ATCC 6919 was seeded on tryptic soy agar (TSA) plates supplemented with 5% sheep's blood. It was incubated at 37 °C and then transferred to Brain Heart Broth (BHI) broth supplemented with 1% dextrose. Using the McFarland 0.5 scale, bacterial inocula were standardized and 100 μL of culture broth (20 μL of extract and 80 μL of inoculum) was added. Doxycycline was the positive control at concentrations of 8 μg/mL to 0.015 μg/mL. The microplates were incubated for 24 h at 37°C and 10 μL of resazurin (0,01%) was added and allowed to incubate at 37°C for 2 h. The blue coloration was interpreted as absence of bacterial growth and the pink coloration as growth. The minimum inhibitory concentration (MIC) was determined to be 500 ug/mL and through UPLC-MS analysis, the major components were found to be Mormordicina IV and Kuguacina S.
ABSTRACT The present study aimed to evaluate the effect of the acetone extract of the leaves of Momordica charantia L. on Cutibacterium acnes. For this purpose, the previously prepared acetone extract was solubilized in 1% dimethylsulfoxide at a concentration of 2000 µg/mL and and diluted to a concentration of 3.91μg/mL. The strain Cutibacterium acnes ATCC 6919 was seeded on tryptic soy agar (TSA) plates supplemented with 5% sheep's blood. It was incubated at 37 °C and then transferred to Brain Heart Broth (BHI) broth supplemented with 1% dextrose. Using the McFarland 0.5 scale, bacterial inocula were standardized and 100 μL of culture broth (20 μL of extract and 80 μL of inoculum) was added. Doxycycline was the positive control at concentrations of 8 μg/mL to 0.015 μg/mL. The microplates were incubated for 24 h at 37°C and 10 μL of resazurin (0,01%) was added and allowed to incubate at 37°C for 2 h. The blue coloration was interpreted as absence of bacterial growth and the pink coloration as growth. The minimum inhibitory concentration (MIC) was determined to be 500 ug/mL and through UPLC-MS analysis, the major components were found to be Mormordicina IV and Kuguacina S.
Link to repository: https://hdl.handle.net/20.500.14414/22476
Discipline: Farmacia
Grade or title grantor: Universidad Nacional de Trujillo. Facultad de Farmacia y Bioquímica
Grade or title: Químico Farmacéutico
Juror: Ruiz Reyes, Segundo Guillermo; Venegas Casanova, Edmundo Arturo; Silva Correa, Carmen Rosa
Register date: 3-Oct-2024
This item is licensed under a Creative Commons License