Efecto antibacteriano in vitro del extracto etanólico de la semilla de persea americana (palta) en cepas de staphylococcus aureus atcc 25923

Abstract

En el presente trabajo se determinó el efecto antibacteriano de la semilla de Persea americana en cepas de Staphylococcus aureus ATCC 25923. Metodología. Se preparó el extracto etanólico de la muestra de Persea americana, para la prueba de solubilidad con diferentes solventes, Screening fitoquímico, preparación de los discos para la incubación, se determinó la concentración óptima del extracto etanólico y su efecto antibacteriano in vitro en cepas de Staphylococcus aureus mediante el método de Kirby Bauer con el fármaco Ciprofloxacino. La investigación es de tipo experimental. Resultados. El extracto contiene compuestos fenólicos, carbohidratos, azúcares reductores, lactonas , insaturadas, con menor cantidad de antocianinas, cantidades mínimas de taninos y saponinas. Se observó un incremento progresivo de los halos de inhibición hasta 13.83 mm a medida que se incrementaba la concentración del extracto etanólico de la semilla de Persea americana presenta un efecto antibacteriano “in vitro” sobre Staphylococcus aureus. Conclusiones. El extracto etanólico de la semilla de Persea americana contiene compuestos fenólicos, carbohidratos, azúcares reductores y lactonas, insaturadas. El extracto presentó efecto antibacteriano “in vitro” sobre la cepa de Staphylococcus aureus ATCC 25923 a concentraciones de elevadas y finalmente el extracto etanólico de la semilla de Persea americana tubo una acción antibacteriana en un 50% en comparación al control positivo de ciprofloxacino.

The in vitro antibacterial effect of the ethanolic extract of the Persea americana seed (avocado) on strains of Staphylococcus aureus ATCC 25923. Methodology. Some secondary metabolites like Phenolic compounds, lactones, reducing sugars and carbohydrates with greater presence in the extract were identified; In addition, the optimal concentration of ethanolic extract and its antibacterial effect in vitro were determined in Staphylococcus aureus strains by means of the agar diffusion technique with the drug Ciprofloxacin. The research is pure experimental with a quantitative approach, the ethanolic extract was prepared, the strains of Staphylococcus aureus were planted and the discotheques were prepared for incubation. Results. The abundant presence of phenolic compounds, carbohydrates, reducing sugars and lactones, unsaturated, lower quantities of anthocyanins and minimum amounts of tannins and saponins, as well as the phenolic compounds that occur in the chromatography and in the capacity of the spectrophotometry. By means of the method of Kirby Bauer it is a progressive change of the halos of inhibition of up to 13.83 mm, a measure that increases in the concentration of the ethanolic extract of the Persea americana seed and finally the ethanolic extract of the Persea seed is checked American present an antibacterial effect "in vitro" on Staphylococcus aureus, but that does not get to be superior to that of ciprofloxacin. Conclusions The abundance of phenolic compounds, carbohydrates, reducing sugars and lactones, unsaturated, could be responsible for the antibacterial activity. The ethanolic extract of the Persea americana seed showed an antimicrobial effect "in vitro" on the strain of Staphylococcus aureus ATCC 25923 and is looking for results statistically and, finally, the ethanolic extract of the Persea americana seed. 50% compared to the positive control of ciprofloxacin.