Bibliographic citations
Ambrosini, R., Sopprani, E. (2021). Efecto de mutaciones en genes distintos a pncA en cepas de Mycobacterium tuberculosis resistentes a pirazinamida que presentan actividad parcial de pirazinamidasa [Universidad Peruana Cayetano Heredia]. https://hdl.handle.net/20.500.12866/9096
Ambrosini, R., Sopprani, E. Efecto de mutaciones en genes distintos a pncA en cepas de Mycobacterium tuberculosis resistentes a pirazinamida que presentan actividad parcial de pirazinamidasa []. PE: Universidad Peruana Cayetano Heredia; 2021. https://hdl.handle.net/20.500.12866/9096
@misc{renati/493682,
title = "Efecto de mutaciones en genes distintos a pncA en cepas de Mycobacterium tuberculosis resistentes a pirazinamida que presentan actividad parcial de pirazinamidasa",
author = "Sopprani Martínez, Emilio",
publisher = "Universidad Peruana Cayetano Heredia",
year = "2021"
}
Tuberculosis is a disease caused by the infection of the bacillus Mycobacterium tuberculosis. Its treatment can be complex due to the existence of strains resistant to firstand second-line drugs such as rifampicin and isoniazid, which are known as multi-drug resistant strains (MDR-TB) (1). (2). Sheen et al. found that at least 30% of MDR-TB cases in Peru show pyrazinamide resistant strains (3). Pyrazinamide (PZA) is a pro-drug used as a first-line treatment for TB by reason of its ability to eliminate latent-phase bacilli. PZA is activated intracellularly, where it is converted into pyrazinoic acid (POA) by the enzyme pyrazinamidase (PZAase). PZA resistance can be caused by many factors; being the loss of PZAse activity by mutations in the pncA gene the principal factor associated to resistance. Quiliano et al. demonstrated that PZAase structure and enzymatic activity are not related to PZA resistance (2). When PZAase activity is nonexistent, the strain is completely PZA resistant; however, partial loss of activity caused by pncA mutations failed to explain resistance by itself, since the conserved PZAse activity is enough to allow the conversion of PZA to POA in these strains (3). The present report intends to evaluate the contribution of the genetic background to the resistance to PZA in strains of Mycobacterium tuberculosis H37Rv which are resistant to PZA and exhibit mutations in pncA but conserve PZAase activity.
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