Bibliographic citations
Benavides, L., (2012). Efecto del Método de Colección y Tensión de Oxígeno sobre el desarrollo embrionario de Ovocitos Bovinos fecundados y cultivados In Vitro [Tesis, Universidad Nacional Mayor de San Marcos]. https://hdl.handle.net/20.500.12672/1571
Benavides, L., Efecto del Método de Colección y Tensión de Oxígeno sobre el desarrollo embrionario de Ovocitos Bovinos fecundados y cultivados In Vitro [Tesis]. PE: Universidad Nacional Mayor de San Marcos; 2012. https://hdl.handle.net/20.500.12672/1571
@misc{renati/493002,
title = "Efecto del Método de Colección y Tensión de Oxígeno sobre el desarrollo embrionario de Ovocitos Bovinos fecundados y cultivados In Vitro",
author = "Benavides Idrogo, Lenin Adolfo",
publisher = "Universidad Nacional Mayor de San Marcos",
year = "2012"
}
--- There are many factors that influence the success of the technique of in vitro embryo production (IVP), many of which are still poorly understood or the influence mechanism has not yet been clarified. The collection method to choose in laboratory conditions and the optimal oxygen tension for better embryo development are still a matter of debate among researchers. The methods used to obtain cultivable oocytes are follicle aspiration and cortex dissection from the ovary, whereas two oxygen tension, 5% and 20%, are commonly used in the embryo production. The objective of this study was to evaluate the influence of the collection method and oxygen tension on PIV parameters (cleavage rate and blastocyst rate). In Experiment 1, the effect of two collection methods, aspiration and dissection, on embryonic development was compared. The highest percentage of cleavage at 48 hours post insemination was obtained with the aspiration method (71.39%), but no difference was observed in the percentage of blastocysts at 7 and 9 days post insemination, additionally it was noted that the number of oocytes recovered per ovary was significantly higher with the dissection method. In Experiment 2, it was compared two oxygen tensions in the culture stage, 5% and 20% oxygen, and it was obtained higher cleavage and blastocyst rate at day 7 post insemination with an 5% oxygen tension. Then, according to these results it can be concluded that follicular aspiration method and a 5% oxygen tension are included within the optimal conditions for the PIV. Keywords: embryo, oxygen, collection, bovine, IVP
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