Bibliographic citations
Giraldo, J., (2012). Producción de ramnolípidos por pseudomonas aeruginosa Pb 25: evaluación de su actividad emulsificante y de remoción de metales pesados [Tesis, Universidad Nacional Mayor de San Marcos]. https://hdl.handle.net/20.500.12672/1413
Giraldo, J., Producción de ramnolípidos por pseudomonas aeruginosa Pb 25: evaluación de su actividad emulsificante y de remoción de metales pesados [Tesis]. PE: Universidad Nacional Mayor de San Marcos; 2012. https://hdl.handle.net/20.500.12672/1413
@misc{renati/491700,
title = "Producción de ramnolípidos por pseudomonas aeruginosa Pb 25: evaluación de su actividad emulsificante y de remoción de metales pesados",
author = "Giraldo Zambrano, Johnny Daniel",
publisher = "Universidad Nacional Mayor de San Marcos",
year = "2012"
}
--- Since the industrial revolution, advances in science and technology have increased the human capacity to exploit natural resources causing ever-increasing disturbances in ecosystems. In this context, the use of biosurfactants represents a promising alternative application for any technological process of remediation of natural environments. The objective of this work was based on the evaluation of the emulsifying activity and the ability to remove heavy metals with a rhamnolipidic biosurfactant produced by Pseudomonas aeruginosa strain PB25, using glycerol as sole carbon source. In this media this strain had a specific growth rate (μ) of 0,0351 h^-1 and a generational time (td) of 19,75 h. At the same time it produced maximum concentration of 2.47 g/L rhamnolipid, with yields (Y) of 0.13 g/g and productivity of 0.082 g/L-h. Similarly, the rhamnolipid is managed to achieve a maximum value of emulsifying activity compared to crude oil from 5.257 UAE/mL and E24 emulsification index of 53, 64, 62 and 84% for crude oil, diesel oil 2, gasoline and kerosene respectively. In addition, the rhamnolipid got to remove 98% of lead and 99% of cadmium in aqueous solutions at pH 11. In conclusion, this type of biosurfactant can be used in future biotechnological processes for the remediation of natural environments contaminated by the presence of heavy metals and/or crude oil and its derivatives. Keywords: Remediation, biosurfactant, rhamnolipid, Pseudomonas aeruginosa, emulsifying activity, hydrocarbons, heavy metals.
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