Bibliographic citations
Vargas, M., (2003). Caracterización de tres cepas de Beauveria brongniartii (Saccardo) Petch y su virulencia en Phthorimaea operculella (Zeller) y Symmetrischema tangolias (Gyen) [Tesis, Universidad Nacional Mayor de San Marcos]. https://hdl.handle.net/20.500.12672/1419
Vargas, M., Caracterización de tres cepas de Beauveria brongniartii (Saccardo) Petch y su virulencia en Phthorimaea operculella (Zeller) y Symmetrischema tangolias (Gyen) [Tesis]. PE: Universidad Nacional Mayor de San Marcos; 2003. https://hdl.handle.net/20.500.12672/1419
@misc{renati/489818,
title = "Caracterización de tres cepas de Beauveria brongniartii (Saccardo) Petch y su virulencia en Phthorimaea operculella (Zeller) y Symmetrischema tangolias (Gyen)",
author = "Vargas Flores, Melisa Elisée",
publisher = "Universidad Nacional Mayor de San Marcos",
year = "2003"
}
--- The objective of this research was to carry out morphological, phisiological and molecular characterization of three isolates of Beauveria brongniartii: CIPCa18(85), CIPCu1(44) y CIPH1(1). In addition, entomogenous activity was tested in Phthorimaea operculella y Symmetrischema tangolias. Isolate CIPCu1(44) showed shorter and spherical conidia while isolates CIPCa18(85) y CIPH1(1) showed tipical elipsoidal conidia. RAPD technique showed a relation between isolates CIPCu1(44) and CIPLM1 (B. bassiana) with 50.4% of similarity. Patogegenicity test showed that isolates were effective to first instar larves, the use of 1 x 109 conidia/ml caused until 100% mortality for both insects. CL50 for isolates CIPCa18(85), CIPCu1(44) and CIPH1(1) obtained in P. operculella larves were 2.71 x 105, 5.53 x 105 and 5.19 x 105 conidias/ml and CL90 for each isolate were 6.81 x 106, 1.22 x 107 and 5.97 x 106 conidias/ml respectively. CL50 for isolates CIPCa18(85), CIPCu1(44) and CIPH1(1) obtained in S. tangolias were 1.16 x 106, 9.37 x 106 and 4.52 x 106 conidias/ml and CL90 for each isolate were 6.01 x 107, 1.46 x 109 and 2.17 x 108 conidias/ml respectively. In other essays, isolates of B. brongniartii needed more time to kill 50% of S. tangolias larves (9 to 10 days) while isolates of B. brongniartii needed only 3 to 5 days to kill 50% of P. operculella. With CL90, isolates needed about 3 days for both insects. Finally, each CL50 y CL90 of three isolates were tested in eggs, last instar larves and pupae, results showed that all develop stages were susceptible to B. brongniartii. Isolate CIPCa18(85) showed the best entomogenous activity to make control of P. operculella y S. tangolias because it came of the same insect order, we suggest more studies with this isolate in greenhouse.
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