Bibliographic citations
Quispe, D., (2009). Perfil molecular de Mycobacterium tuberculosis en muestras biológicas del tracto respiratorio inferior de pacientes limeños con sospecha de tuberculosis [Tesis, Universidad Nacional Mayor de San Marcos]. https://hdl.handle.net/20.500.12672/885
Quispe, D., Perfil molecular de Mycobacterium tuberculosis en muestras biológicas del tracto respiratorio inferior de pacientes limeños con sospecha de tuberculosis [Tesis]. PE: Universidad Nacional Mayor de San Marcos; 2009. https://hdl.handle.net/20.500.12672/885
@misc{renati/482521,
title = "Perfil molecular de Mycobacterium tuberculosis en muestras biológicas del tracto respiratorio inferior de pacientes limeños con sospecha de tuberculosis",
author = "Quispe Huamanquispe, Dora Graciela",
publisher = "Universidad Nacional Mayor de San Marcos",
year = "2009"
}
Tuberculosis is an infectious disease that constitutes a serious public health problem worldwide, mainly in developing countries such as Peru. The limitations of the traditional methods of diagnosis (smear and culture) as well as the high incidence of this disease have created the need to implement new strategies to increase the sensitivity of tests to reduce time to establish a confirmed diagnosis. Since there are not many researches at the region of the Lower Respiratory Tract (LRT), the objective of this study was to determine the molecular profile of Mycobacterium tuberculosis in biological samples taken from the LRT of patients with suspicion tuberculosis. In the present study, 43 samples from patients with clinical suspicion of tuberculosis were evaluated. The samples were obtained through bronchial lavage, bronchial aspirate, bronchial secretions, bronco-alveolar lavage, and sucked bronco-alveolar broncofibroscopy. DNA extraction was prepared from each sample, and it was used in a PCR- Nested test targeting the gene encoding the protein A (65KDa) from M. tuberculosis. The results showed that DNA from M. tuberculosis was detected in 77% of the processed samples, revealing a high sensitivity and specificity of this method. From the positive samples, 82% corresponded to those obtained from the bronchial tree top region. This last result is explained because of the fact that most of the samples were taken directly from this region, which is the starting point for the mycobacteria dissemination toward other regions of the respiratory tract. In addition, the application of the PCR-Nested test increased the sensitivity of detection by 5.5 fold compared to a single event amplification, demonstrating the usefulness of this test in the analysis of biological material with low mycobacterial load as those used in this work. Finally, we conclude that the PCR-Nested test is a highly sensitive and specific method for detecting DNA from Mycobacterium tuberculosis in samples from the LRT.
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