Bibliographic citations
Sulca, M., (2010). Identificación y caracterización de integrones y su asociación con la resistencia a antibióticos en cepas de Vibrio spp. aisladas de ambientes marinos contaminados de Lima-Perú [Tesis, Universidad Nacional Mayor de San Marcos]. https://hdl.handle.net/20.500.12672/3326
Sulca, M., Identificación y caracterización de integrones y su asociación con la resistencia a antibióticos en cepas de Vibrio spp. aisladas de ambientes marinos contaminados de Lima-Perú [Tesis]. PE: Universidad Nacional Mayor de San Marcos; 2010. https://hdl.handle.net/20.500.12672/3326
@mastersthesis{renati/479845,
title = "Identificación y caracterización de integrones y su asociación con la resistencia a antibióticos en cepas de Vibrio spp. aisladas de ambientes marinos contaminados de Lima-Perú",
author = "Sulca Lopez, Marcos Alejandro",
publisher = "Universidad Nacional Mayor de San Marcos",
year = "2010"
}
--- The research aimed to incorporate a quick identification methodology known as ARDRA (Amplified Ribosomal DNA Restriction Analysis) to identify Vibrio species. Technique was standardized with reference strains. Then, bacterial strains were isolated associated with the cultivation of Litopenaeus vannamei. Subsequently, biochemical tests were performed to find candidate strains belonging to the genus Vibrio. Upon completion of this first stage, the standard technique (ARDRA) was applied for candidate strains, thus confirming the feasibility of the method under the conditions studied. In a second step, the 16S rDNA region sequenced to confirm and identify candidate strains for phylogenetic analysis. Three different species were reported with high similarity belonging to the Vibrio core group (Vibrio communis, Vibrio harveyi and Vibrio parahaemolyticus). With these results, it was possible to design a quick identification by ARDRA to identify the Vibrio core group and Vibrio communis. The design methodology ARDRA was supported by a bioinformatics diagnostic assessment, obtaining this evaluation, a sensitivity and specificity of 97,1 and 76.9% respectively for the identification of Vibrio core group, while identifying the species Vibrio communis, yielded a sensitivity and specificity of 100 and 97,4%, respectively. Finally, it has proved possible to identify certain Vibrio species associated with aquaculture Litopenaeus vannamei, by ARDRA identification and this methodology has the advantage of being much faster and cheaper compared with the identification by phylogenetic analysis, having in turn, the disadvantage of being dependent on the use of the sequencing at first for ARDRA design. Keywords: Litopenaeus vannamei, ARDRA, Vibrio communis, Vibrio harveyi, Vibrio parahaemolyticus, Vibrio core group, bioinformatic diagnostic evaluation.
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