Bibliographic citations
Pomar, G., (2002). Tuberización in vitro de Oxalis tuberosa Mol. “Oca“ como una alternativa para la producción de tubérculo semilla [Tesis, Universidad Nacional Mayor de San Marcos]. https://hdl.handle.net/20.500.12672/1399
Pomar, G., Tuberización in vitro de Oxalis tuberosa Mol. “Oca“ como una alternativa para la producción de tubérculo semilla [Tesis]. PE: Universidad Nacional Mayor de San Marcos; 2002. https://hdl.handle.net/20.500.12672/1399
@misc{renati/477656,
title = "Tuberización in vitro de Oxalis tuberosa Mol. “Oca“ como una alternativa para la producción de tubérculo semilla",
author = "Pomar Vela, Gerardo Manuel",
publisher = "Universidad Nacional Mayor de San Marcos",
year = "2002"
}
An adequate protocol was developed to obtain in vitro microtubers of Oxalis tuberosa “oca“. At first the plantlets were grown in the micropopagation medium M-10 (MS medium containing vitamins, myo-inositol, pantothenic acid, GA, NAA and BAP). After 5 – 6 weeks the propagation medium was changed by the tuber induction media tested (White medium and 13 times diluted White medium containing both 8 % sucrose, vitamins, myo-inositol and several concentrations of BAP and CCC) and maintained in continuous darkness. It was also tested tuberization response when the apical buds were cutted. Different accessions of Oxalis tuberosa have had different behaviors for the same culture medium. The more uniform response was found in the medium containning BAP between 0.1 and 0.25 ppm supplied with CCC 200 ppm. Depending on the accession The White basic medium can be diluted or not. Finally, the buds excision resulted a good mechanic system to induce the tuberization, giving us higher weight and number of microtubers.
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