Bibliographic citations
Bezada, S., (2010). Efecto Estrogénico del extracto Hidroalcóholico de alfalfa (Medicago Sativa) en Ratas Albinas Ovariectomizadas [Tesis, Universidad Nacional Mayor de San Marcos]. https://hdl.handle.net/20.500.12672/2598
Bezada, S., Efecto Estrogénico del extracto Hidroalcóholico de alfalfa (Medicago Sativa) en Ratas Albinas Ovariectomizadas [Tesis]. PE: Universidad Nacional Mayor de San Marcos; 2010. https://hdl.handle.net/20.500.12672/2598
@misc{renati/331780,
title = "Efecto Estrogénico del extracto Hidroalcóholico de alfalfa (Medicago Sativa) en Ratas Albinas Ovariectomizadas",
author = "Bezada Quintana, Sandra Gracia",
publisher = "Universidad Nacional Mayor de San Marcos",
year = "2010"
}
Objective: Demonstrate the estrogenic properties of hydroalcoholic extract of the species Medicago sativa L (alfalfa) in albino rats ovariectomized (OVX). Methods: We used 48 Sprague Dawley female albino rats of 200 to 250 g. 8 weeks of age, of which 40 underwent surgical removal of both ovaries (OVX) by means of bilateral ovariectomy on the flank. After post-operative rats were divided into six groups and provided the hydroalcoholic extract of Medicago sativa (alfalfa) under the following work schedule: Group 1: Negative Control (OVX), vehicle, VO 2 ml / kg, Group 2: Positive Control (OVX), estradiol, SC, 3ug/kg; Group 3 (OVX) Alfalfa extract, VO, 100 mg / kg, Group 4 (OVX), Alfalfa extract, VO, 500 mg / kg, Group 5: (OVX), Alfalfa extract, VO, 1000 mg / kg, Group 6: (No OVX), Control of the surgical procedure. The treatment lasted 14 days. The outcome measures were uterine weight, body weight, estrous cycle changes by vaginal smear and hormonal profile analysis. Results: The increase in uterine weight at doses of 500mg/kg and 1000mg/kg., Besides the presence of alkaloids, flavonoids and saponins in the hydroalcoholic extract of alfalfa in regular amount. In the surgical technique used anesthetic combination xylazine (2mg/kg), ketamine (40mg/kg) induced an optimal surgical plane (plane 2), without complications or postoperative death of animals. Conclusions: The hydroalcoholic extract of Medicago sativa L (alfalfa) causes an oestrogenic effect by increasing the uterus weight in OVX rats at doses of 500mg/kg and 1000mg/kg. It also contains saponins in regular amount. The surgical technique for bilateral ovariectomy flank is a practical, reliable and less traumatic for the animals that can do research on estrogen deficiency.
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