Bibliographic citations
Aguilar, B., (2015). Elucidación estructural del polisacárido del alga roja Porphyra columbina y determinación in vivo de la capacidad antioxidante e hipolipemiante [Tesis, Universidad Nacional Mayor de San Marcos]. https://hdl.handle.net/20.500.12672/4287
Aguilar, B., Elucidación estructural del polisacárido del alga roja Porphyra columbina y determinación in vivo de la capacidad antioxidante e hipolipemiante [Tesis]. PE: Universidad Nacional Mayor de San Marcos; 2015. https://hdl.handle.net/20.500.12672/4287
@misc{renati/331208,
title = "Elucidación estructural del polisacárido del alga roja Porphyra columbina y determinación in vivo de la capacidad antioxidante e hipolipemiante",
author = "Aguilar Velasquez, Blanca Yessenia",
publisher = "Universidad Nacional Mayor de San Marcos",
year = "2015"
}
The main aim of this thesis is the structural elucidation of polysaccharide from the red seaweed Porphyra columbina, hot water extracted, purified and lyophilized, and the study of its antioxidant activity in mice and the lipid-lowering effect in Rabbits. Prior acute oral toxicity was evaluated considering the biological material would be exposed to different concentrations of the same. The IR and 1H-NMR spectrum shows that the polysaccharide of P. columbina has a typical porphyran structure: β-D-galactose, 4-O-α-L-galactose-6-sulphate or 3,6-anhydro-α-L-galactose units. The toxicity study for the case of acute oral toxicity shown to be nontoxic up to 2000 mg/Kg strength, and for repeated dose 28-day oral toxicity study to 50 mg/kg liver damage is evident. In vivo tests in mice measured the activities of antioxidant enzymes superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) as well as the mean levels of malondialdehyde (MDA). The results show increase of the 3 enzymes tested also decreased MDA by intraperitoneal administration dose-dependent polysaccharide. Hypolipidemic effect in rabbits was determined by the evaluation of the lipid profile: measurement of C, TG, HDL and LDL and VLDL was calculated. The results were statistically significant, p < 0.05, for both lipids and lipoproteins in assessment compared with control group of lipemia. Body weight of the rabbits showed significant differences, p < 0.05, for the groups in treatment. Keywords: Porphyra, porphyrano, lipid peroxidation, antioxidant, hypolipidemic, lipoprotein.
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