Bibliographic citations
Porturas, D., (2011). Efectividad de la asociación cetrimida-clorhexidina 15% / 0.15% frente a clorhexidina 2% en la erradicación de biofilms de Enterococcus faecalis [Tesis, Universidad Nacional Mayor de San Marcos]. https://hdl.handle.net/20.500.12672/2195
Porturas, D., Efectividad de la asociación cetrimida-clorhexidina 15% / 0.15% frente a clorhexidina 2% en la erradicación de biofilms de Enterococcus faecalis [Tesis]. PE: Universidad Nacional Mayor de San Marcos; 2011. https://hdl.handle.net/20.500.12672/2195
@misc{renati/329199,
title = "Efectividad de la asociación cetrimida-clorhexidina 15% / 0.15% frente a clorhexidina 2% en la erradicación de biofilms de Enterococcus faecalis",
author = "Porturas Araujo, Djasmin",
publisher = "Universidad Nacional Mayor de San Marcos",
year = "2011"
}
The purpose of this study was to determine the effectiveness of the association of Cetrimide-Chlorhexidine 15%/0.15% with Chlorhexidine 2% in the eradication of biofilms of Enterococcus faecalis in vitro. The used methodology for this aim was the creation of biofilms in glass slides for 48 hours of incubation at 37 ºC, in aerobic conditions from Enterococcus faecalis ATCC 29212 strains in BHI infusion. They were subjected to the tested irrigants during the periods of 1, 3 and 5 minutes, respectively. There were two groups of independent experiments and a negative control with saline solution in three repetitions for each irrigant and time tested. The tested irrigants were Cetrimide/ Chlorhexidine 15%/015%, (SAFEBLON) , Chlorhexidine 2% and physiological saline. After the contact with the irrigants , biofilms were cultivated in TSA agar and incubated during 24 hours at 37 ºC for the quantification of the UFC/mL and determine the percentage of bacterial inhibition and consequently the percentage of the irrigants effectiveness. The results obtained show that the obtained percentage of bacterial inhibition of Enterococcus faecalis biofilms with Cetrimide - Chlorhexidine (15%/0.15%) was 99,99% ; with Chlorhexidine (2%), was at 100% and with Physiological Saline (negative control) ranged between 24% and 31%; during three tested times : 1,3 and 5 minutes. Therefore, It concluded that there is no significant difference in the eradication of Enterococcus faecalis biofilms; by the application of the association Cetrimide-Chlorhexidine (15%/ 0.15%) and Chlorhexidine 2% at any of the three tested times; allowing to use indistinctly less concentration of Chlorhexidine from 2% to 0.15% by the adjuvant use of Cetrimide 15%. Keywords: Cetrimide, surfactant, biofilms, Enterococcus faecalis, surface tension
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