Bibliographic citations
Ortiz, C., (2004). Aplicación de la técnica del ADN polimorfico amplificado al azar (RAPD) en el estudio molecular de Lama pacos [Tesis, Universidad Nacional Mayor de San Marcos]. https://hdl.handle.net/20.500.12672/2334
Ortiz, C., Aplicación de la técnica del ADN polimorfico amplificado al azar (RAPD) en el estudio molecular de Lama pacos [Tesis]. PE: Universidad Nacional Mayor de San Marcos; 2004. https://hdl.handle.net/20.500.12672/2334
@misc{renati/325805,
title = "Aplicación de la técnica del ADN polimorfico amplificado al azar (RAPD) en el estudio molecular de Lama pacos",
author = "Ortiz Alfaro, Conrad",
publisher = "Universidad Nacional Mayor de San Marcos",
year = "2004"
}
-- The Random Amplified Polymorphic DNA (RAPD) is applied on the molecular study of Lama pacos (Alpaca), this methodology is proposed because the microsatellites and DNA mitochondrial techniques, which are used commonly, don't have a practical utility when is necessary to analyze great quantity of samples. Genomic DNA obtained from total blood and primers of 10 nucleotides were used to standardize the RAPD technique following the standardized protocols, and concentration of MgCl2 was modified at 2.5 mM which allowed an increase at the intensity and a better visualization of the bands. After the standardization, the identification of informative primers was realized, beginning with 23 primers, selecting only 7 (OPF 05, OPI 04, OPB 03, OPI 18, OPB 11, OPA 18 and OPI 14), because the presence of several bands for the analyzed samples. These primers let obtain different profiles between hybrid and pure alapacas; in the sample were not found alpacas with a similar bands profiles to those presented by the pure animals, this would explain the hybridization grade that exists due to an inadequate care in the crossing, this results in, for example a low quality in the fiber production from the hybrid alpacas and llamas, diminishing the economic entrance for the rural families. In 8 alpacas the primers OPB-03 and OPA-18 showed polymorphic bands of 650 pb and 1000 pb respectively, this polymorphism could indicate some mutation or intraespecific variability, since these animals don't present evident phenotypic differences.
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