Bibliographic citations
Pletickosich, J., (2019). Adherencia de tejido conectivo en discos de titanio mecanizado grado III® implantados en ratas cepa albina sprague dawley Cusco - 2019 [Tesis, Universidad Andina del Cusco]. https://hdl.handle.net/20.500.12557/3277
Pletickosich, J., Adherencia de tejido conectivo en discos de titanio mecanizado grado III® implantados en ratas cepa albina sprague dawley Cusco - 2019 [Tesis]. PE: Universidad Andina del Cusco; 2019. https://hdl.handle.net/20.500.12557/3277
@misc{renati/25957,
title = "Adherencia de tejido conectivo en discos de titanio mecanizado grado III® implantados en ratas cepa albina sprague dawley Cusco - 2019",
author = "Pletickosich Cuba, Jhovissa",
publisher = "Universidad Andina del Cusco",
year = "2019"
}
The purpose of the investigation was to determine the adhesion of connective tissue to grade III® mechanized titanium discs implanted in Albina Sprague dawley strain rats of the Automated Bioterium of the Andina University of Cusco. For which an alive experimental design was followed. The sample consisted of 11 rats, 2 rats for the pilot test and 20 grade III® machined titanium discs. The rats were housed in transparent polycarbonate boxes of 40x35x22 centimeters provided with filter covers, pressurized, with water intake, the bed was covered with sterilized pine wood chips. Purified water and Purina brand balanced feed was administered in the Sterilization Laboratory. The environment maintained a temperature of 23-25 ° C and humidity between 46-48%, the Smart Flow team maintained the pressurization of the internal air of the boxes. A 200mg / ml of ketamine hydrochloride solution was prepared in a tuberculin syringe with a 25-27G gauge of 1⁄2 to 1 inch with a small bevel. An intraperitoneal puncture was performed in approximately 3-5 minutes to observe the anesthetic effect. An incision was made at the side of the shoulder blade with a catheter. Then, the area was enlarged and the titanium disc was inserted and closed with a stitch. Then, according to the time established in the experiment, disk was removed and placed in 10% formalin for transport. A cardiac puncture was done to obtain blood and each rat was sacrificed according to the time of the experiment. The sacrifice was due to the extension and depth of tissue removed next to the implant to perform the histopathological study, which was incompatible with life. The results reflected adherence of: fibroblasts, macrophages and lymphocytes. Synthesis of collagen fibers and the distribution of the elements were organized in a circumscribed manner to the titanium disc. The values also reflected that there is no infection or allergic reaction, but giant platelets were found, which participate in blood clot formation and vessel repair.
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