Bibliographic citations
Mamani, C., (2018). Mapa físico de polimorfismos de nucleótido simple en Alpaca (Vicugna pacos) usando un panel de células híbridas irradiadas Alpaca/Hámster [Tesis, Universidad Nacional Agraria La Molina]. https://hdl.handle.net/20.500.12996/3592
Mamani, C., Mapa físico de polimorfismos de nucleótido simple en Alpaca (Vicugna pacos) usando un panel de células híbridas irradiadas Alpaca/Hámster [Tesis]. : Universidad Nacional Agraria La Molina; 2018. https://hdl.handle.net/20.500.12996/3592
@mastersthesis{renati/247316,
title = "Mapa físico de polimorfismos de nucleótido simple en Alpaca (Vicugna pacos) usando un panel de células híbridas irradiadas Alpaca/Hámster",
author = "Mamani Mondragón, Camilo Vicente",
publisher = "Universidad Nacional Agraria La Molina",
year = "2018"
}
The aim of this research study was to develop a physical map of single nucleotide polymorphisms (SNPs) in alpaca using the alpaca/hamster radiation hybrid panel and a Bovine high-density SNP genotyping microarray. The specific objectives were: a) to identify SNPs on the bovine microarray present only in alpaca DNA and absent in hamster DNA; b) to determine the distances between SNP groups co-inherited in the alpaca/hamster radiation hybrid panel and; c) to determine the chromosomal location of the SNP linked groups. The 96 DNA samples present in the panel were genotyped (92 hybrid cells clones, 2 alpaca samples, 1 hamster sample, 1:10 alpaca/hamster sample) and were evaluated with the GenomeStudio, R and Excel programs. The SNPs found in alpacas were analyzed with the Carthagene software to identify linkage groups. These linked groups were located in the VicPac 2.0.2 database using the Blast software and the ShortBlast command with the help of the Galaxy platform. A total of 50,686 SNPs from the bovine microarray were detected in the alpaca genome and absent in the hamster genome. Thirty-three linkage groups that include 216 SNPs were identified. Their calculated distances that ranged between 65.3 and 671.9 cR, with a LOD> 3.0. The sequence of 31 of these SNPs was found in the VicPac 2.0.2 and the location of 3 SNPs (1BovineHD0100027068, 8BovineHD0600011684 and 13BovineHD1100019948) was inferred to be on alpaca chromosomes 1, 2 and 4.
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