Bibliographic citations
Cabezas, O., (2023). Caracterización morfológica, patológica y molecular de Pyricularia sp. causante de manchas foliares en Calathea sp. en Huánuco [Universidad Nacional Agraria La Molina]. https://hdl.handle.net/20.500.12996/6525
Cabezas, O., Caracterización morfológica, patológica y molecular de Pyricularia sp. causante de manchas foliares en Calathea sp. en Huánuco []. PE: Universidad Nacional Agraria La Molina; 2023. https://hdl.handle.net/20.500.12996/6525
@mastersthesis{renati/242978,
title = "Caracterización morfológica, patológica y molecular de Pyricularia sp. causante de manchas foliares en Calathea sp. en Huánuco",
author = "Cabezas Huayllas, Oscar Esmael",
publisher = "Universidad Nacional Agraria La Molina",
year = "2023"
}
An unreported Pyricularia specie had been causing serious damages in the production of Calathea sp., also known as wira bijao, which are employed in the elaboration of many typical dishes in the Peruvian Amazonia region. In this study, this causal agent was characterized was culturally, morphologically, pathologically, and molecularly characterized in comparison with Pyricularia oryzae isolated from rice. The morphological characterization was made by measuring its conídia and conidiophores, which were obtain directly from an infected sample and monosporic isolated on PDA. The culture characterization was made by comparing the rate of growth of mycelial, by varying types of cultivation, levels of pH and lighting conditions. Meanwhile, the pathological characteristics were evaluated by cross-inoculation of isolated Pyricularia - bijao and isolated Pyricularia – rice, on Calathea sp. and Oryza sativa’s seedling produced in plant nursery. And the molecular identification was made by the sequencing and analysis of the genetic length of 28S gen’s fragments (D1/D2 region) and internal transcribed spacer (ITS). The morphologic and culture results of this unreported specie show a highly level of similarity to the Pyricularia genre’s characteristics. The dimensions of the isolated Pyricularia – bijao’s conídia (27.60±1.51 x 10.7±0.84 µm) are 33.0 percentage larger, while its conidiophores dimensions (102.89±15.28 x 4.11±0.39 µm) are 15.1 percentage shorter, both in comparison to the Pyricularia oryzae isolated from rice. In other hand, its rate of growth on PDA, 0.282 cm/day, is 2.3-fold slower than the isolated Pycularia - rice. The cross-inoculation results show that the Pyricularia that infects rice may cause minor damages on bijao, while the Pyricularia that infects bijao does not cause any visible damage on rice, but it does in other four species of Calathea. The results of molecular analysis concluded that the causal agent of leaf scorch on Calathea sp. is the Pyricularia oryzae Cavara 1882, which is the first report of an infection in a host that does not belong to any species of the Poaceae family.
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