Bibliographic citations
Gonzales, G., (2023). Susceptibilidad al mal de altura en bovinos criollos y Brown Swiss determinada mediante constantes hematológicas y marcadores genéticos [Universidad Nacional Agraria La Molina]. https://hdl.handle.net/20.500.12996/5856
Gonzales, G., Susceptibilidad al mal de altura en bovinos criollos y Brown Swiss determinada mediante constantes hematológicas y marcadores genéticos []. PE: Universidad Nacional Agraria La Molina; 2023. https://hdl.handle.net/20.500.12996/5856
@phdthesis{renati/242739,
title = "Susceptibilidad al mal de altura en bovinos criollos y Brown Swiss determinada mediante constantes hematológicas y marcadores genéticos",
author = "Gonzales Aparicio, Gonzalo Wladimir",
publisher = "Universidad Nacional Agraria La Molina",
year = "2023"
}
The main objective of the research was to analyze the susceptibility to altitude sickness in Creole and Brown Swiss cattle raised in hypoxic conditions due to altitude in the highlands of Peru, through their hematological constants and genetic markers, for which the hematological constants of Creole cattle (CRIZA) and Brown Swiss cattle (BSZA) raised in the Andes of Peru between 3,213 and 4,309 masl were studied and compared to cattle with brisket disease (BSMA) and to Brown Swiss raised at low altitude, between 243 and 1,306 masl (BSZB). A correlation of 0.92 between hemoglobin (Hb) and hematocrit (Ht) was observed. In addition, it was observed that the values of Ht, red blood cell count (RGR) and white blood cell count (RGB), among the upper decile of the BSZA and CRIZA groups, were lower than those observed in BSMA cattle (p<0.01). Then, based on the Hb results, 60 cattle were identified and grouped into Brown Swiss with high Hb (BSHbA) (n=13), Brown Swiss with low Hb (BSHbB) (n=11), creoles with high Hb (CRHbA) (n=13), creoles with low Hb (CRHbB) (n=12), in addition to BSMA cattle (n=11). Based on the sequence analysis of 41 bovine genomes from five breeds, available from the Sequence Read Archive (SRA) of the National Center for Biotechnology Information (NCBI), we identified SNPs and developed PCR primers for the exonic sequences of the EPAS, NOS, VEGFA and EPO genes. After extracting the DNA from the sampled animals, the exonic regions of these genes were amplified and sequenced by the Sanger methodology. Ten single nucleotide polymorphisms (SNPs) were identified in these exonic regions. However, no specific associations were found between the SNPs and the groups of animals studied. Only one SNP located in the intronic position Cr.19: 19403681T>C of the NOS2 gene showed a different frequency between Creole cattle and Brown Swiss (P<0.05), in addition, said SNP was found linked to two other located in exon 12 of the same gene.
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