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Title: 3D reconstruction of the cerebellar germinal layer reveals tunneling connections between developing granule cells
Advisor(s): Khare, Harshavardhan; Valdivia Silva, Julio Ernesto
OCDE field: https://purl.org/pe-repo/ocde/ford#2.11.00
Issue Date: 5-Apr-2023
Institution: Universidad de Ingeniería y Tecnología
Abstract: The difficulty of retrieving high-resolution, in vivo evidence of the proliferative and migratory processes occurring in neural germinal zones has limited our understanding of neurodevelopmental mechanisms. Here, we used a connectomic approach using a high-resolution, serial-sectioning scanning electron microscopy volume to investigate the laminar cytoarchitecture of the transient external granular layer (EGL) of the developing cerebellum, where granule cells coordinate a series of mitotic and migratory events. By integrating image segmentation, three-dimensional reconstruction, and deep-learning approaches, we found and characterized anatomically complex intercellular connections bridging pairs of cerebellar granule cells throughout the EGL. Connected cells were either mitotic, migratory, or transitioning between these two cell stages, displaying a chronological continuum of proliferative and migratory events never previously observed in vivo at this resolution. This unprecedented ultrastructural characterization poses intriguing hypotheses about intercellular connectivity between developing progenitors and its possible role in the development of the central nervous system.
Note: Artículo sustentado el 12 de Diciembre 2023 para la obtención del título profesional de Bioingeniería.
Discipline: Bioingeniería
Grade or title grantor: Universidad de Ingeniería y Tecnología. Bioingeniería
Grade or title: Bioingeniera
Juror: Saavedra Espinoza, Harry Gustavo; Deza Figueroa, Manuel Arturo; Agapito Cordova, Felix Alonso
Register date: 15-Dec-2023



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