Bibliographic citations
Lozano, P., (2025). Resistencia antibiótica de bacterias causantes de mastitis subclínica bovina en el distrito de Huanchaco de la provincia de Trujillo, 2023 [Tesis, Universidad Privada Antenor Orrego]. https://hdl.handle.net/20.500.12759/57511
Lozano, P., Resistencia antibiótica de bacterias causantes de mastitis subclínica bovina en el distrito de Huanchaco de la provincia de Trujillo, 2023 [Tesis]. PE: Universidad Privada Antenor Orrego; 2025. https://hdl.handle.net/20.500.12759/57511
@misc{renati/1339314,
title = "Resistencia antibiótica de bacterias causantes de mastitis subclínica bovina en el distrito de Huanchaco de la provincia de Trujillo, 2023",
author = "Lozano Ñique, Piero Alexandre",
publisher = "Universidad Privada Antenor Orrego",
year = "2025"
}
The objective of this study was to evaluate the antibiotic resistance of bacteria causing bovine subclinical mastitis in the district of Huanchaco, province of Trujillo, in 2023. For this purpose, 131 milk samples were collected from cows diagnosed with subclinical mastitis, which were stored at 4°C in sterile jars until they were analyzed in the Veterinary Microbiology laboratory of the Veterinary Medicine and Animal Husbandry study program of the Antenor Orrego Private University. Bacterial isolation was performed using the streak technique on Blood agar and MacConkey agar, incubating at 37°C for 24 hours. Subsequently, the respective biochemical tests were carried out for bacterial identification, resulting in: Staphylococcus aureus 31.15% (19/61), Streptococcus agalactiae 16.39% (10/61), Escherichia coli 34.43% (21/61) and Klebsiella spp. 18.03% (11/61). 73.7% (14/19) of Staphylococcus aureus and 80% (8/10) of Streptococcus agalactiae showed resistance to Penicillin G, while 76.2% (16/21) of Escherichia coli strains and 72.7% (8/11) of Klebsiella spp. showed resistance to Cephalotin. In general, most of the bacterial strains evaluated showed high levels of resistance to β-lactams, with resistances ranging from 9.5% to 80%, depending on the species and the specific type of antibacterial agent used. In the case of Tetracyclines, values from 45.5% to 68.4% are shown; and for Aminoglycosides, Quinolones and Diaminopyrimidines/Sulfonamides from 5.3% to 54.5%. In addition, the prevalence of multidrug-resistant strains of Streptococcus agalactiae, Escherichia coli, Klebsiella spp and Staphylococcus aureus resistant to more than three antibiotics is shown, with 70%, 57%, 55% and 47%, respectively. By applying Fisher's statistical test, it was found that S. aureus strains present a significant association with amikacin, penicillin G, amoxicillin/clavulanic acid and tetracycline; S. agalactiae with amikacin, penicillin G and amoxicillin/clavulanic acid; E. coli with amoxicillin/clavulanic acid, cephalotin and tetracycline; and no association was found between Klebsiella spp strains and the resistance rate
This item is licensed under a Creative Commons License