Bibliographic citations
Plasencia, S., (2019). Presentación clínica, epidemiología molecular y sensibilidad antimicrobiana de Acinetobacter baumannii en muestras de pacientes provenientes del Instituto Nacional de Enfermedades Neoplásicas (INEN) [Tesis, Universidad Peruana de Ciencias Aplicadas (UPC)]. http://hdl.handle.net/10757/648790
Plasencia, S., Presentación clínica, epidemiología molecular y sensibilidad antimicrobiana de Acinetobacter baumannii en muestras de pacientes provenientes del Instituto Nacional de Enfermedades Neoplásicas (INEN) [Tesis]. PE: Universidad Peruana de Ciencias Aplicadas (UPC); 2019. http://hdl.handle.net/10757/648790
@misc{renati/1287036,
title = "Presentación clínica, epidemiología molecular y sensibilidad antimicrobiana de Acinetobacter baumannii en muestras de pacientes provenientes del Instituto Nacional de Enfermedades Neoplásicas (INEN)",
author = "Plasencia Rebata, Stefany",
publisher = "Universidad Peruana de Ciencias Aplicadas (UPC)",
year = "2019"
}
Background: Acinetobacter baumannii, an important nosocomial opportunistic pathogen worldwide, causes ventilator-associated pneumonia as well as bloodstream infections in critically ill patients, with mortality rates between 10 and 43%. A. baumannii expresses several resistance mechanisms, including the production of β-lactamases, changes in cellular membrane and production of efflux pumps. These latter transport toxic and antimicrobial compounds out of the bacterial membrane and represent an important factor for development of multidrug resistance. The aim of this study is to evaluate efflux pump expression in Acinetobacter baumannii strains from a tertiary care hospital in Lima, Peru between 2014 and 2016. Methods: Acinetobacter spp. were identified by BD Phoenix ™ automated system and A. baumannii strains were detected by amplification of the blaOXA-51 gene by PCR. Antimicrobial susceptibility to fluoroquinolones and aminoglycosides was assessed by broth microdilution technique, according to CLSI guidelines. The activity of the efflux pumps system was assessed by the addition of phenylalanine inhibitor of arginine-β-naphthylamide (PAβN). Results: A total of nineteen Acinetobacter baumannii strains were included in the study. All were positive for the blaOXA-51 gene by PCR and had clinical information associated. The samples were non-duplicate and collected from different sources. A total of eight strains (42,1%) demonstrated an increase in the susceptibility rates and sixteen (84,2%) expressed efflux pumps. Conclusion: Decreasing antimicrobial resistance rates against quinolones and aminoglycosides was demonstrated by the addition of the PABN efflux pump inhibitor. Efflux pumps still have an important role in the emergence of multi-drug resistant strains of A. baumannii and may be useful as a complementary therapy for this pathogen.
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