Bibliographic citations
Llallire, D., Quiroz, C. (2024). Efecto de diferentes métodos de descongelación sobre determinados parámetros bioquímicos en muestras de suero [Universidad Peruana Cayetano Heredia]. https://hdl.handle.net/20.500.12866/16593
Llallire, D., Quiroz, C. Efecto de diferentes métodos de descongelación sobre determinados parámetros bioquímicos en muestras de suero []. PE: Universidad Peruana Cayetano Heredia; 2024. https://hdl.handle.net/20.500.12866/16593
@misc{renati/1281993,
title = "Efecto de diferentes métodos de descongelación sobre determinados parámetros bioquímicos en muestras de suero",
author = "Quiroz Diaz, Carla Xiomara",
publisher = "Universidad Peruana Cayetano Heredia",
year = "2024"
}
Introduction: Currently, the relationship between serum and storage temperature continues to be studied, however, there is little information on thawing methods andtheir impact on the final results of each analyte. Objectives: To determine the effectof different thawing methods on certain biochemical parameters in serum samples. Materials and methods: Observational, cross-sectional study, the sample size was75 serum samples obtained from 15 persons, we obtained 5 cryovials for each one,one of the cryovials was processed immediately, two were stored at 4°C and there maining two were stored at -20°C; two days later the cryovials were taken to 25°Cand to a water bath (37°C). The biochemical parameters evaluated were glucose,creatinine, aspartate aminotransferase, uric acid and total bilirubin. Statistical analysis was used with the Medcalc v22.0.23 program using repeated test Anova,to determine if it was statistically significant (p<0.05) and if they had a percentagebias variation described by Westgard. Results: Glucose, uric acid and total bilirubin (stored at -20°C and 4°C) subjected to the different thawing methods suffered notable alterations in relation to their basal average, although a statistically significant difference was also found in all the analytes evaluated (p<0. 001. 001);furthermore, a percentage bias difference described by Westgard was only found inglucose and total bilirubin (stored at -20°C and 4°C), when brought to 25°C andwater bath (37°C); and like wise, in creatinine (stored at -20°C) when thawed atwater bath (37°C). Conclusions: Both statistically significant variation and a difference in percent bias described by Westgard were appreciated in glucose and total bilirubin when brought to 25°C and water bath (37°C).
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