Bibliographic citations
Mendoza, L., (2010). Aislamiento y selección de hongos lipolíticos a partir de aceites vegetales de desecho (proveniente de frituras) utilizados en la elaboración de biodiesel [Tesis, Universidad Nacional Mayor de San Marcos]. https://hdl.handle.net/20.500.12672/872
Mendoza, L., Aislamiento y selección de hongos lipolíticos a partir de aceites vegetales de desecho (proveniente de frituras) utilizados en la elaboración de biodiesel [Tesis]. PE: Universidad Nacional Mayor de San Marcos; 2010. https://hdl.handle.net/20.500.12672/872
@misc{renati/1213218,
title = "Aislamiento y selección de hongos lipolíticos a partir de aceites vegetales de desecho (proveniente de frituras) utilizados en la elaboración de biodiesel",
author = "Mendoza Canales, Luis Ignacio",
publisher = "Universidad Nacional Mayor de San Marcos",
year = "2010"
}
--- Most vegetable oils used for frying foods then become waste oils (used edible oils).This waste product untreated is discharged usually on rivers, canals or the sea causing contamination. The treatment of waste oils with lipolytic microorganisms capable of the bioconversion these oils in products of interest such Biodiesel, is recent and little studied. Therefore, in this work were isolated lipolytic fungi from 6 samples of waste vegetable oil from frying. Two methods were used: one of them corresponds to the methodology standardized by the American Public Health Association (APHA), but modified for the purposes of this research and was called: modified APHA Method, and the other one using the minimum salt Czapek Dox (3% lipid substrate) that is called: Czapek Method. The ability to produce lipases was tested “in vitro” when cultivated on Czapek Agar (pH 6.3) with 3% lipid substrate as sole carbon source: Test of Lipolysis (PL). 123 strains were isolated, of these 105 were lipolitic fungi: 55 got with the modified APHA Method and 50 with Czapek Method. Of these, only 9.52% (10 strains) were able to complete degradation of the lipid substrate Czapek Agar (3%). 8 belonging to the genus Penicillium, 1 to Aspergillus and 1 to Geotrichum. The remaining showed degradation halo low to 1 mm and diameter of colonies less than 0.5 cm. In the modified APHA Method using petroleum ether as diluent alternative influenced to isolate smaller number strains than with the use of phosphate buffer. However, with the ether was isolated from two of the three genera of fungi with greater capacity for lipolysis (Penicillium and Geotrichum). There weren’t significant differences when used ether as an additional diluent in Czapek Method, only the genus Penicillium isolated within the group of the most lipolytic reported in this investigation. Key words: lipolytic fungi, lipase, waste oil, modified APHA Method, Czapek Method, petroleum ether
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