Bibliographic citations
Gonzales, I., (2025). Búsqueda de oligopéptidos quelantes de Hierro usando una biblioteca aleatoria de Plásmidos construida en Sccharomyces cerevisiae [Universidad Nacional Agraria La Molina]. https://hdl.handle.net/20.500.12996/6898
Gonzales, I., Búsqueda de oligopéptidos quelantes de Hierro usando una biblioteca aleatoria de Plásmidos construida en Sccharomyces cerevisiae []. PE: Universidad Nacional Agraria La Molina; 2025. https://hdl.handle.net/20.500.12996/6898
@misc{renati/1117216,
title = "Búsqueda de oligopéptidos quelantes de Hierro usando una biblioteca aleatoria de Plásmidos construida en Sccharomyces cerevisiae",
author = "Gonzales Medina, Ingrid Carolay",
publisher = "Universidad Nacional Agraria La Molina",
year = "2025"
}
Saccharomyces cerevisiae is a widely used model organism due to its ease of genetic manipulation and its ability to integrate large DNA fragments, as well as to construct errorfree plasmids through homologous recombination. In this study, this ability was exploited to develop a plasmid library that allowed the production of random oligopeptides. An in vivo cloning strategy in S. cerevisiae was applied for the construction of the plasmid library and additionally, a staining assay was implemented for the identification of the iron-binding oligopeptides. The staining assay used potassium ferrocyanide, which reacts with ferric ions to form an intense blue pigment, known as Prussian blue. This assay allowed the identification of yeast transformants that, by producing a chelating oligopeptide, accumulate greater amounts of the metal. With this procedure, two oligopeptides with potential iron chelating activity were identified.
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