Bibliographic citations
Cabrera, P., (2021). Integridad de la membrana citoplasmática en semen refrigerado y congelado de toros usando dos dilutores sintéticos [Universidad Nacional Agraria La Molina]. https://hdl.handle.net/20.500.12996/5365
Cabrera, P., Integridad de la membrana citoplasmática en semen refrigerado y congelado de toros usando dos dilutores sintéticos []. PE: Universidad Nacional Agraria La Molina; 2021. https://hdl.handle.net/20.500.12996/5365
@misc{renati/1116895,
title = "Integridad de la membrana citoplasmática en semen refrigerado y congelado de toros usando dos dilutores sintéticos",
author = "Cabrera Montes, Pedro Luis",
publisher = "Universidad Nacional Agraria La Molina",
year = "2021"
}
Four Simmental and Braunvieh bulls (2 of each breed) from the Donoso Experimental Station of MINAGRI were used. Semen samples were extracted for processing (refrigeration and freezing) at The National Sperm Bank and the effect of the synthetic dilutors Optixcell and Andromed on the integrity of the cytoplasmic membrane was evaluated. Sperm concentration, pH, motility, vitality and cytoplasmic membrane integrity were analyzed using hypoosmotic solution (100 mlOsm/L). For statistical analysis, the Minitab statistical package was used using a completely randomized block design with dilutors as treatments and each bull breed as blocks. A DBCA was applied for sperm motility, vitality and HOST characteristics, for refrigerated and thawed semen in eight replicates. In the evaluation of pure sperm, the means 3.34±1.42 ml in volume, 1442.40±327.34 million spermatozoa/ml of concentration, 3.8 of mass motility, 7 of pH and creamy white color were obtained. In the ANOVA results for the refrigerated semen; in the endosmosis test (HOST), 48.97±15.23 % and 39.66±13.39% were obtained using Andromed and Optixcell respectively, finding significant differences (P<0.05). In relation to the thawed sperm, 41.15±10.26% and 26.82±7.30% of endosmosis were obtained with Optixcell and Andromed respectively, also finding significant differences (P<0.05). Therefore, it is concluded that the Optixcell dilutor improved the conservation of the integrity of the cytoplasmic membrane of spermatozoa. With respect to the breeds, significant differences (P<0.05) were found in motility and vitality in refrigerated and thawed sperm. However, in the HOST test, no statistical differences (P≥0.05) were found in both refrigerated and thawed sperm.
This item is licensed under a Creative Commons License