Bibliographic citations
Lobo, D., (2024). Construcción de dos bibliotecas de plásmidos para la síntesis y secreción de oligopéptidos aleatorios o semialeatorios en S. cerevisiae [Universidad Nacional Agraria La Molina]. https://hdl.handle.net/20.500.12996/6576
Lobo, D., Construcción de dos bibliotecas de plásmidos para la síntesis y secreción de oligopéptidos aleatorios o semialeatorios en S. cerevisiae []. PE: Universidad Nacional Agraria La Molina; 2024. https://hdl.handle.net/20.500.12996/6576
@misc{renati/1115638,
title = "Construcción de dos bibliotecas de plásmidos para la síntesis y secreción de oligopéptidos aleatorios o semialeatorios en S. cerevisiae",
author = "Lobo Reyes, Deisy Kevelin",
publisher = "Universidad Nacional Agraria La Molina",
year = "2024"
}
Although all oligopeptides are formed by a few amino acids (2 to 20), they are diverse and have many important applications. Importantly, it is possible to produce them to allow investigation of their properties using a variety of techniques, including the construction of plasmid libraries. In turn, the synthesized oligopeptides might have different destinations, for example, inside the cell, to be secreted into the extracellular space or anchored on the cell surface. Thus, once these libraries have been obtained, a search for the desired oligopeptides is carried out through screening strategies. This thesis describes the production and secretion of a great variety of oligopeptides, using the yeast Saccharomyces cerevisiae. The random and semi-random fragments to be cloned were obtained via an optimized PCR strategy that allowed their efficient and reproducible generation. An in vivo cloning strategy was applied for the assembly of the expression plasmids and library construction, achieving high cloning efficiencies (62.5 to 70 %).
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