Bibliographic citations
Leiva, S., (2022). Diversidad genética de Trichoderma como agente biocontrolador de la moniliasis (Moniliophthora roreri) para la producción sostenible del cacao nativo [Universidad Nacional Agraria La Molina]. https://hdl.handle.net/20.500.12996/5609
Leiva, S., Diversidad genética de Trichoderma como agente biocontrolador de la moniliasis (Moniliophthora roreri) para la producción sostenible del cacao nativo []. PE: Universidad Nacional Agraria La Molina; 2022. https://hdl.handle.net/20.500.12996/5609
@phdthesis{renati/1114674,
title = "Diversidad genética de Trichoderma como agente biocontrolador de la moniliasis (Moniliophthora roreri) para la producción sostenible del cacao nativo",
author = "Leiva Espinoza, Santos Triunfo",
publisher = "Universidad Nacional Agraria La Molina",
year = "2022"
}
The present study aimed to study the diversity of Trichoderma species present in the cocoa agroecosystem of Amazonas, Peru, and its potential as an in vitro and in vivo biocontroller of frosty pod rot (Moniliophthora roreri), hereinafter referred to as CM. Molecular and morphological identification of 234 Trichoderma isolates was carried out to determine their in vitro biocontrol potential on MC and finally to determine their biocontrol potential in the final field. Molecular identification included analysis of ITS, tef1, rpb2 sequences subjected to the pairwise similarity comparison protocol, supported by morphological identification. For in-vitro biocontrol evaluation, using a DCA, we evaluated mycoparasitism, antibiosis and finally potential antagonism was calculated as an indicator for selection of candidates for efficient biocontrol of MC at field level, where we evaluated the in-vivo effect of four isolates, using a DBCA design and epidemiological variables, performance and biocontrol efficacy. Nineteen species of the genus Trichoderma were identified, five of which are first reported for Peru and five species correspond to new species: in addition, it was determined that T. afroharzianum was the most abundant species. On the other hand, the results observed for mycoparasitism and antibiosis against M. roreri support the existence of a high intra- and interspecific variability in Trichoderma. Eighteen species had isolates with high in vitro antagonism potential (more than 50 por ciento). It is shown that the in vitro evaluation of the biocontrol potential of Trichoderma strains, even within the same species, is variable and should be evaluated by integrating the levels of mycoparasitism and antibiosis, in the form of potential antagonism. Finally, the four Trichoderma strains applied in the field obtained superior values in incidence reduction with direct impact on yield increase. The strains CP24-6 and F14M3, with 27 por ciento and 38 por ciento, reached the lowest levels of FPR incidence; besides an efficiency of 62 por ciento.
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