Bibliographic citations
Huarcaya, M., (2024). Aislamiento y selección de bacterias productoras de Lipasas nativas altamente selectivas sobre triglicéridos [Universidad Nacional Agraria La Molina]. https://hdl.handle.net/20.500.12996/6897
Huarcaya, M., Aislamiento y selección de bacterias productoras de Lipasas nativas altamente selectivas sobre triglicéridos []. PE: Universidad Nacional Agraria La Molina; 2024. https://hdl.handle.net/20.500.12996/6897
@misc{renati/1114601,
title = "Aislamiento y selección de bacterias productoras de Lipasas nativas altamente selectivas sobre triglicéridos",
author = "Huarcaya Calderón, Margaret Melissa",
publisher = "Universidad Nacional Agraria La Molina",
year = "2024"
}
Enrichment of polyunsaturated fatty acids, particularly ω-3 fatty acids, is a strategy adopted to enhance the nutritional value of oils. Among the various methodologies, enzymatic hydrolysis via lipases has garnered substantial interest within the oil industry. However, the limited availability of lipases necessitates the discovery of more efficient novel enzymes, preferably of microbial origin. In this study, bacterial strains were isolated from the intestines of fish and screened using natural and synthetic ester substrates. Ten strains with potential lipolytic activity were preselected through qualitative assessments, with the genus Staphylococcus being predominant, as identified by 16S rRNA gene sequencing. Of the identified strains, LMB-06 (S. urealyticus), LMB-Ju02 (S. urealyticus), LMB-JH06 (S. epidermidis) and LMB-JH07 (S. warneri) demonstrated high lipolytic activity. LMB-06 and LMB-Ju02 were selected to produce lipolytic extracts due to their superior activity in nitrogen-rich media. The crude extracts containing lipases were partially characterized. The optimal pH for the lipases was 7 with optimal temperatures of 30°C for LMB-06 and 37°C for LMB-Ju02. Enzymatic activity increased in the presence of Ca2+, while inhibitors such as EDTA and hydrogen peroxide reduced activity. Additionally, both lipases contained in the crude extracts exhibited stability in organic solvents such as hexane and ethanol, which also enhanced their lipolytic activity. When applied to the hydrolysis of various oils, the lipases from LMB-06 and LMB-Ju02 effectively hydrolyzed olive, sunflower, palm, and fish oils, achieving hydrolysis rates of 2.9% and 17.6% for fish and olive oils, respectively. Genome sequencing and subsequent analysis revealed the presence of one lipase gene in both LMB-06 and LMB-Ju02, eight in LMB-JH06, and three in LMB-JH07. In conclusion, the isolated bacterial strains exhibit significant potential for the hydrolysis and esterification of triglyceride-containing oils. This enzymatic capability could substantially improve both the nutritional and economic value of fish oils produced in Peru.
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