Bibliographic citations
Matsubara, J., (2010). Diversidad fenotípica y molecular de bacterias simbióticas del cultivo de pallar (Phaseolus lunatus L.) en el Valle de Supe - Barranca [Tesis, Universidad Nacional Agraria La Molina]. https://hdl.handle.net/20.500.12996/874
Matsubara, J., Diversidad fenotípica y molecular de bacterias simbióticas del cultivo de pallar (Phaseolus lunatus L.) en el Valle de Supe - Barranca [Tesis]. : Universidad Nacional Agraria La Molina; 2010. https://hdl.handle.net/20.500.12996/874
@misc{renati/1114589,
title = "Diversidad fenotípica y molecular de bacterias simbióticas del cultivo de pallar (Phaseolus lunatus L.) en el Valle de Supe - Barranca",
author = "Matsubara Bautista, Jorge Minoru",
publisher = "Universidad Nacional Agraria La Molina",
year = "2010"
}
´Pallar´ or 'Lima bean' (Phaseolus lunatus L.) is the second most economically important crop between Phaseolus species, in Peru, since its role in gastronomic and pre-Hispanic cultural heritage and the increasing intemational appreciation. It is nodulated by nitrogen fixing bacteria broadly called rhizobia. The aim of this study was to isolate and to characterize strains from P. lunatus nodules with different phenotypic and molecular methods for the subsequent selection and use as biofertilizers in the same crop. Bacteria were isolated from nodules collected from fields of P. lunatus in the Supe valley in the north-central coast of Peru. Ali isolates were characterized on their cultural characteristics, physiology and plant growth promotion capabilities, while 18 selected strains were characterized in their symbiotic performance, intrinsic antibiotic resistance, rep-PCR fingerprinting and 16S rDNA RFLP (ARDRA) and sequence. Toree isolates were Rhizobium and 60 were Bradyrhizobium. The latter group was sub-divided into 26 alkalinizating and 34 extra-alkalinizating strains according to the change the pH of YEM media All isolates grew at 37 ºC and were acid- and alkaline-tolerant. Sensitivity to 8° and 40ºC was showed by extra-alkalinizating bradyrhizobia and rhizobia, respectively. The latter were salt-tolerant and were better IAA producers and tri-calcic phosphate solubilizators than the former. Alkalinizating and extra-alkalinizating selected bradyrhizobia were resistant to nalidixic acid and cefixime, respectively. And both did to lincomycin. Rhizobia were resistant to penicillin. All the selected strains nodulated P. lunatus and six of them showed a significant increase (p>0.5) in the dry weight of the aerial part. Sorne strains could nodulate P. vulgaris and Vigna unguiculata. By BOX-, ERIC-, and REP-PCR, there were distinguished 10 separate banding patterns, while combining the three methods; there were 11 groups from 18 strains. Sequencing the 16S rRNA gene revealed, and in agreement with ARDRA results, that rhizobia were phylogenetically related to R. alamii, and alkalinizating and extra-alkalinizating bradyrhizobia belong to B. yuanmingense aod to a new linage of Bradyrhizobium, respectively.
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