Bibliographic citations
Lázaro, R., (2015). Determinación de la presencia de sustancias antinutricionales y alérgenos en semilla y torta de sacha inchi (Plukenetia volubilis L.) [Tesis, Universidad Nacional Agraria La Molina]. https://hdl.handle.net/20.500.12996/1822
Lázaro, R., Determinación de la presencia de sustancias antinutricionales y alérgenos en semilla y torta de sacha inchi (Plukenetia volubilis L.) [Tesis]. : Universidad Nacional Agraria La Molina; 2015. https://hdl.handle.net/20.500.12996/1822
@misc{renati/1114505,
title = "Determinación de la presencia de sustancias antinutricionales y alérgenos en semilla y torta de sacha inchi (Plukenetia volubilis L.)",
author = "Lázaro Aguilar, Rina Dionisia",
publisher = "Universidad Nacional Agraria La Molina",
year = "2015"
}
This study was aimed to know the nutritional potential of the seeds and residues of extraction of the oil ("cake") of the species Plukenetia volubilis by determining the presence trypsin inhibitors, lectins, allergenic protein fractions that present reactivity crossed with peanuts and / or soybean, and the presence of ricin-type peptide, specifically the A chain of ricin. Hemagglutination activity was observed even in the dilution 1/32 cake and 1/64 Sacha inchi seed. The activity of trypsin inhibitors (ATI) in seed and cake was 31.9 and 46.19 UTI/mg, respectively. In electrophoretic separation Sacha inchi protein on SDS-PAGE protein bands detected at 10, 12, 15, 17, 25, 30, and 52 ~ kDa. In immunoblotting assays, no reaction was observed with sera of allergic patients peanut or soybean. On the other hand, reaction is observed between soy and peanuts, using serum from patients allergic to soy, in peanut protein fractions 38, 40 and 42 ~ kDa. The anti-ricin A chain antibody recognizes proteins in the protein fractions of 30-32 kDa and seed Sacha Inchi cake. In conclusion, hemagglutination activity and moderate activity trypsin inhibitors found; and a potential epitope of the A chain of ricin nontoxic seed protein fractions and Sacha inchi cake, but these did not show cross-reactivity with sera of allergic patients peanut or soybean.
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