Bibliographic citations
Mascco, J., (2023). Cuantificación de fructooligosacáridos por HPLC-RID e inulina mediante UV-VIS en productos de yacón (Smallanthus Sonchifolius) provenientes de dos regiones del Perú de diferentes altitudes. [Universidad Nacional Agraria La Molina]. https://hdl.handle.net/20.500.12996/5712
Mascco, J., Cuantificación de fructooligosacáridos por HPLC-RID e inulina mediante UV-VIS en productos de yacón (Smallanthus Sonchifolius) provenientes de dos regiones del Perú de diferentes altitudes. []. PE: Universidad Nacional Agraria La Molina; 2023. https://hdl.handle.net/20.500.12996/5712
@misc{renati/1113623,
title = "Cuantificación de fructooligosacáridos por HPLC-RID e inulina mediante UV-VIS en productos de yacón (Smallanthus Sonchifolius) provenientes de dos regiones del Perú de diferentes altitudes.",
author = "Mascco Rosales, Jackelin Lizeth",
publisher = "Universidad Nacional Agraria La Molina",
year = "2023"
}
Yacón (S. sonchifolius) is a native plant in the Andes recognized for being a rich source of fructooligosaccharides and inulin whose nutritional benefits are reduction of blood glucose, control of harmful bacteria in the intestine, calcium and magnesium absorption and the prevention of colon cancer. The principal aim of this research was the quantification of fructooligosaccharides by HPLC-RID and inulin using UV-VIS spectrophotometry in yacon products such as flour, syrup, and juice from Bagua, Amazonas (429 m.s.n.m.) and Pachitea, Huánuco (3040m.s.n.m.) For the validation of the fructooligosaccharides by HPLC-RID method, the European guide for validation of analytical methods and the Brazilian legislation were taken into consideration. The results showed that retention times values for kestose and nystose standards were 10,2 and 13,4 minutes and showed a linearity of 0,991 and 0,982, respectively. The limits of detection (LOD) and quantification (LOQ) of kestose were 0.9611 and 3.2035 mg/mL, while values of 0,3355 and 1,1182 mg/mL were obtained in nystose. Likewise, these fructooligosaccharides had a recovery of 109,57 ± 3,44 and 88,47 ± 4,74 (%) respectively and an intraday and interday precision of 2,57 and 2,4 for kestose and 3,05 and 5,53 for the case of nystose. In the case of the validation of inulin using UV-VIS spectrophotometry, a linearity of 0,985 was obteined. The limits of detection (LOD) and quantification (LOQ) were 0,0309 and 0,0102 mg/mL. Also, presented a recovery of 112,43 ± 4,6 and an intraday and interday precision of 7,73 and 6,5, respectively. Plants from both altitudes were genetically similar using SRAP markers. However, the chemical composition of the products from both places presented differences due to geographical location, temperature, and harvest time. Finally, this research determined that the concentration of fructooligosaccharides was higher in Pachitea products having its syrup as the product with the highest content. In relation to inulin, the juice from Pachitea was the product with the highest concentration.
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