Bibliographic citations
Zegarra, A., (2023). Evaluación del desarrollo embrionario en ovocitos bovinos madurados in vitro con la adición de antioxidante y moduladores de cAMP [Universidad Nacional Agraria La Molina]. https://hdl.handle.net/20.500.12996/6387
Zegarra, A., Evaluación del desarrollo embrionario en ovocitos bovinos madurados in vitro con la adición de antioxidante y moduladores de cAMP []. PE: Universidad Nacional Agraria La Molina; 2023. https://hdl.handle.net/20.500.12996/6387
@misc{renati/1113403,
title = "Evaluación del desarrollo embrionario en ovocitos bovinos madurados in vitro con la adición de antioxidante y moduladores de cAMP",
author = "Zegarra Paredes, Angelica Milagros",
publisher = "Universidad Nacional Agraria La Molina",
year = "2023"
}
Addition of antioxidants and cAMP modulators during in vitro maturation (IVM) may play a decisive role in preimplantation embryonic development. Antioxidants protect cells from free radicals and the effects of ROS due to oxidative stress. cAMP modulators are the regulators to maintain meiotic arrest by suppressing the activation of maturation-promoting factor (MPF). Therefore, the objective of this study was to evaluate bovine embryonic development from oocytes matured in vitro with the addition of antioxidant and cAMP modulators. In the experiment I, to determine the level of anthocyanin that maximizes the increase in quantity and quality of blastocysts developed on day 7, 462 COCs (n=462) were recovered from slaughterhouse and were placed intro five treatments with different levels of anthocyanin (0, 1, 10, 20 and 40 uM). In experiment II, to evaluate the effect of cAMP modulators supplemented in IVM, 181 COCs were placed into four pre-IVM treatments for 2 h (control, 1 mM dbcAMP, 0.5 mM IBMX, 1.0 mM dbcAMP + 0.5 mM IBMX), after that the COCs were transferred to IVM medium. for 22 hours to complete in vitro maturation. Subsequently, they were fertilized for 18 h and cultured for seven days under conditions of 5 % CO2 at 38°C. The evaluation of the treatments was carried out in three stages of embryonic development; day 3 (cleavage rate), day 5 (morula rate) and day 7 (blastocyst rate). Data were processed with analysis of variance (ANOVA) and analysis of means (Tukey's test) p < 0.05 using IBM SPSS Statistics 2.0 for Windows. The results of the groups with anthocyanin supplementation and cAMP modulators showed no statistical differences (p < 0.05) in cleavage and blastocyst rate; although, the addition of 1 μM anthocyanin (28.8 %) and 1 mM dbcAMP (34.7 %) in the IVM media showed the highest blastocyst rates. In conclusion, supplementation of anthocyanins and cAMP modulators does not improve the cleavage rate and blastocysts in cattle, requiring complementary studies at the cellular and gene expression level.
This item is licensed under a Creative Commons License