Bibliographic citations
Herrera, S., (2022). Efecto inductivo in vitro de citoquininas sobre el brotamiento de segmentos nodales de palo de rosa (Aniba rosiodora Ducke) [Universidad Nacional de San Martín. Fondo Editorial]. http://hdl.handle.net/11458/4494
Herrera, S., Efecto inductivo in vitro de citoquininas sobre el brotamiento de segmentos nodales de palo de rosa (Aniba rosiodora Ducke) []. PE: Universidad Nacional de San Martín. Fondo Editorial; 2022. http://hdl.handle.net/11458/4494
@misc{renati/1056160,
title = "Efecto inductivo in vitro de citoquininas sobre el brotamiento de segmentos nodales de palo de rosa (Aniba rosiodora Ducke)",
author = "Herrera Pinedo, Silvia Kareli",
publisher = "Universidad Nacional de San Martín. Fondo Editorial",
year = "2022"
}
The present research work was carried out at the Biotechnology Laboratory of the National Institute for Agrarian Innovation (INIA), located in the district of Juan Guerra, in the San Martin region. The objective was to evaluate the inductive effect of three sources and concentrations of cytokinins on the sprouting of Brazilian rosewood (Aniba rosiodora) nodal segments under in vitro conditions. Vegetative segments and M&S culture medium supplemented with: T1 (KIN - 0.45 μM); T2 (KIN - 2.32 μM); T3 (KIN - 23.23 μM); T4 (BAP- 0.45 μM); T5 (BAP - 2.32 μM); T6 (BAP - 23.23 μM); T7 (2Ip - 0.45 μM); T8 (2Ip - 2.32 μM); T9 (2Ip - 23.23 μM) were used. They were distributed in totally randomized designs with a 3A (Sources) x 3B (Concentrations) factorial arrangement and a control treatment. Ten treatments and eight replicates were obtained. In order to reduce the incidence of pathogenic microorganisms in the culture medium, preventive applications were made in the field with a fungal solution at a rate of 1 gL-1, 30 days before each extraction, together with the addition of Vitrofural at the culture medium level; the segments were sterilized with a solution of 2% NaClO + tween 80 for 20 minutes. The data obtained were submitted to analysis of variance and the Tukey multiple range test (p < 0.01). It was determined that by applying preventive applications in the field 30 days before in vitro installation, a survival rate of more than 88 % was reached. Likewise, T5 (BAP- 2.32 μM) and T6 (BAP-23.23 μM) reached a higher number of shoots and high averages with respect to shoot length, obtaining a shoot length of (8.41 mm and 6.13 mm) respectively. Finally, it was identified that T9 (2Ip - 23.23 μM) and T8 (2Ip - 2.32) optained better average in callus formation with 1.2 callus. It is concluded, that the BAP cytokinin source induces greater shoot activation in Brazilian rosewood timber species at the in vitro level.
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