Bibliographic citations
Carvallo, M., (2023). Evaluación fitoquímica, capacidad antioxidante y fenoles totales de las especies Piper reticulatum L. (Cordoncillo acorazonado) y Piper hispidum Sw. (Cordoncillo negro) [Universidad Nacional de San Martín. Fondo Editorial]. http://hdl.handle.net/11458/5601
Carvallo, M., Evaluación fitoquímica, capacidad antioxidante y fenoles totales de las especies Piper reticulatum L. (Cordoncillo acorazonado) y Piper hispidum Sw. (Cordoncillo negro) []. PE: Universidad Nacional de San Martín. Fondo Editorial; 2023. http://hdl.handle.net/11458/5601
@misc{renati/1056142,
title = "Evaluación fitoquímica, capacidad antioxidante y fenoles totales de las especies Piper reticulatum L. (Cordoncillo acorazonado) y Piper hispidum Sw. (Cordoncillo negro)",
author = "Carvallo del Aguila, Maria Albertina",
publisher = "Universidad Nacional de San Martín. Fondo Editorial",
year = "2023"
}
Phytochemical evaluation, antioxidant capacity and total phenols of Piper reticulatum L. (Cordoncillo acorazonado) and Piper hispidum Sw. (Cordoncillo negro) species. The biological and chemical properties conferred to the Piper genus converted it one of the most important genera of the Piperaceae family within the wild flora for its medicinal properties, which have been the reason for conducting multiple investigations of its most representative species. However, despite the great importance of this genus, there are still unstudied species, so this research aims to contribute with chemical and biological results of two of its species, Piper reticulatum L. and Piper hispidum Sw. which are abundant in the Peruvian Amazon, but with scarce verified scientific information. In order to achieve these results, the following objectives were established: to perform the phytochemical evaluation to identify the possible presence of secondary metabolites responsible for the biological activity, to determine the antioxidant capacity and to perform the quantification of total phenols. For these purposes, the leaves of both species were collected and hydroalcoholic extracts were obtained by maceration with 80% ethanol, obtaining yields of 10.012 ± 0.02% for Piper reticulatum L. and 8.938 ± 0.04% for Piper hispidum Sw. In order to achieve these results, the following objectives were established: to perform the phytochemical evaluation to identify the possible presence of secondary metabolites responsible for the biological activity, to determine the antioxidant capacity and to perform the quantification of total phenols. For these purposes, the leaves of both species were collected and hydroalcoholic extracts were obtained by maceration in 80% ethanol, obtaining yields of 10.012 ± 0.02% for Piper reticulatum L. and 8.938 ± 0.04% for Piper hispidum Sw. The phytochemical evaluation was carried out by means of preliminary phytochemical marching using staining and precipitation reactions, for which the presence of tannins, amino acids, flavonoids, quinones, alkaloids and steroids was postulated. Subsequently, the phytochemical analysis was reinforced through chromatographic profiles by UHPLC, identifying referentially the presence of certain polyphenols by comparing UV-Vis spectra obtained with those reported in the literature, thus determining signals for: flavonoids, flavones and phenolic acids in the species Piper reticulatum L. and phenolic acids, flavones and isoflavones in the species Piper hispidum Sw. By means of the DPPH (2.2-diphenyl-1-picrylhydrazyl) method, the antioxidant capacity showed an IC50 of 3.09 mg extract/ml methanol for Piper hispidum Sw. compared to an IC50 of 3.513 mg extract/ml methanol for Piper reticulatum L. The quantification of total phenols was performed by the Folin Ciocalteu method, obtaining for the Piper reticulatum L. species 413.511 ± 3.02 mg GAE/100g of dry extract, compared to 323.622 ± 12.47 mg GAE/100g of dry extract for Piper hispidum Sw. species. It is concluded that both species possess great biological and chemical potential of interest with respect to other species of the genus Piper.
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