Bibliographic citations
Perez, L., (2020). Análisis metagenómico de la comunidad bacteriana del glaciar Pastoruri (Parque Nacional Huascarán, Perú), asociada a un entorno extremófilo [Universidad Nacional de Trujillo]. https://hdl.handle.net/20.500.14414/20348
Perez, L., Análisis metagenómico de la comunidad bacteriana del glaciar Pastoruri (Parque Nacional Huascarán, Perú), asociada a un entorno extremófilo []. PE: Universidad Nacional de Trujillo; 2020. https://hdl.handle.net/20.500.14414/20348
@misc{renati/1050816,
title = "Análisis metagenómico de la comunidad bacteriana del glaciar Pastoruri (Parque Nacional Huascarán, Perú), asociada a un entorno extremófilo",
author = "Perez Palomino, Luis Miguel",
publisher = "Universidad Nacional de Trujillo",
year = "2020"
}
The retracement of the Pastoruri glacier (Huascaran National Park, Peru) is exposing sulfur-rich lithological groups that contaminate the waters with heavy metals and making the pH decrease, producing an acid rock drainage process (ARD), where some extremophilic bacteria inhabit and act as a catalyst, accelerating oxidation and acidification processes. Therefore, the objective of this work was determine the metagenomics bacterial community composition of the Pastoruri glacier, associated with an extremophile environment. To do this, six sampling points corresponding to small water sources between the Huascarán National Park and the Pastoruri glacier were determined, in which the physical-chemical parameters of the water were measured in situ; subsequently, the water and sediment samples were collected, the metagenomic DNA was extracted, the amplification and sequencing of the V4 region of the 16S rRNA gene was made using the IonTorrent PGM technique; then, the sequences were processed by computer for the determination of the operative taxonomic units (OTUs); finally, for the statistical analysis and the graphing of results, the free software RStudio was used. 515787 reads were obtained grouped in 2682 bacterial OTUs and distributed in 11 Phyla (> 1%), finding two common Phyla for all sampling sites corresponding to Proteobacteria (68.54%) as the most abundant, and Acidobacteria (20.95%). Likewise, the bacterial genera that showed predominant relative abundances were Acidiphilium (39.08%), Nitrosovibrio (19.43%), Acidithrix (24.94%), Cyanobacterium (11.97%), Geobacter (14.27%), Rhodoferax (13.71%), Granulicella (10.90%) and Ferrovum (13.22%).
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