Bibliographic citations
Sánchez, D., (2023). Escherichia coli productoras de betalactamasas de espectro extendido aisladas en urocultivos de pacientes del Hospital Provincial de Casas – La Libertad – 2021 [Universidad Nacional de Trujillo]. https://hdl.handle.net/20.500.14414/20719
Sánchez, D., Escherichia coli productoras de betalactamasas de espectro extendido aisladas en urocultivos de pacientes del Hospital Provincial de Casas – La Libertad – 2021 []. PE: Universidad Nacional de Trujillo; 2023. https://hdl.handle.net/20.500.14414/20719
@mastersthesis{renati/1047882,
title = "Escherichia coli productoras de betalactamasas de espectro extendido aisladas en urocultivos de pacientes del Hospital Provincial de Casas – La Libertad – 2021",
author = "Sánchez Tisnado, Denis Andree",
publisher = "Universidad Nacional de Trujillo",
year = "2023"
}
In this investigation, "the prevalence of extended-spectrum beta-lactamase (BLEE)-producing Escherichia coli, isolated from patients with ITU treated at the Hospital de Cascas, La Libertad, from January to July 2021" was determined. Urine samples were inoculated onto blood agar (AS) and Mac Conkey agar (AMC) using the calibrated loop method (1 ul), then incubated at 37°C for 24 to 48 hours. Subsequently, cultures with macroscopic characteristics for E. coli were taken into consideration, with a colony count greater than 105 UFC/mL, then the differential biochemical identification of E. coli was performed. Once the E. coli species had been identified, the isolated colonies were inoculated on Trypticase Soy Agar (TSA) and incubated at 37°C for 24 h to obtain pure cultures, from which the inoculum similar to tube N° 0.5 of the Mac Farland scale (1.5 x 108 CFU/mL), and seeded on Müeller Hinton agar plates. In order to detect E. coli BLEE, the Jarlier method (Committee of the French Society for Microbiology) was used, using discs with antimicrobials such as Amoxicillin/Clavulanic Acid (20/10 μg), cefotaxime (30 μg), ceftazidime (30 μg), aztreonam (30 μg), and ceftriaxone (30 μg). Obtaining as a result 16 E. coli BLEE cultures (16.0%), where 5 cultures (32.25%) were isolated from male patients and 11 cultures (68.75%) from female patients, regarding the age group it was determined that the group between 61 and 80 years has a higher prevalence for E. coli BLEE with 6 (37.5%) cultures, regarding the antibacterials used, it was determined that E. coli BLEE was identified in greater prevalence with the antimicrobials of aztreonan and Cefotaxime with 8 (8 %) cultures for each antimicrobial. Concluding that the prevalence of E. coli. (BLEE), isolated in urine cultures of patients at the Cascas Hospital from January to July 2021 was 16.0%.
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