Bibliographic citations
Abanto, Y., Rojas, A. (2024). Caracterización morfológica, fisicoquímica, mecánica y térmica de celulosa bacteriana producida por Komagataeibacter xylinus empleando semilla de palta como sustrato [Universidad Nacional de Trujillo]. https://hdl.handle.net/20.500.14414/21581
Abanto, Y., Rojas, A. Caracterización morfológica, fisicoquímica, mecánica y térmica de celulosa bacteriana producida por Komagataeibacter xylinus empleando semilla de palta como sustrato []. PE: Universidad Nacional de Trujillo; 2024. https://hdl.handle.net/20.500.14414/21581
@misc{renati/1045591,
title = "Caracterización morfológica, fisicoquímica, mecánica y térmica de celulosa bacteriana producida por Komagataeibacter xylinus empleando semilla de palta como sustrato",
author = "Rojas Llamoga, Angie Estefany",
publisher = "Universidad Nacional de Trujillo",
year = "2024"
}
ABSTRACT The present research work was carried out with the objective of obtaining bacterial cellulose (CB) by Komagataeibacter xylinus from the hydrolyzate of avocado seed powder (SPP). The agroindustrial waste was previously selected, peeled, cut into pieces and dried at 38 °C for 2 days. After that, they were crushed in a mill and sieved in order to unify the particle size. An extraction with ethanol (96%) was carried out on the crushed residue, to eliminate all non-structural or extractive compounds. The agroindustrial residue free of extractives was subjected to acid hydrolysis with 4.23% sulfuric acid and was subsequently centrifuged; The liquid fraction or supernatant resulting from centrifugation was used to quantify reducing sugars by UV-Vis Spectrophotometry (51.16 g/L), and identify structural sugars by HPLC (glucose, maltose, xylose and mannose). CB membranes were produced in Hestrin–Schramm (HS) medium, in which sucrose was used as a carbon source. Likewise, modified Hestrin–Schramm culture medium (HSM) was used, replacing sucrose with powdered avocado seed hydrolyzate (SPP) to produce said membranes. The culture conditions were for 10 days at 30 °C in static conditions, then the samples were dried at 45 °C in an oven for 24 h until constant weight. The volumetric yield and production rate of the CB membranes in the HS and HSM media were determined, and the percentage of reducing sugars (AR) consumed in both media was calculated. The obtained CB-HS and CB-HSM membranes were characterized by moisture content, density, Scanning Electron Microscopy (SEM), X-ray Diffractometry (XRD), Fourier Transform Infrared Spectroscopy (FTIR), Mechanical Analysis, Analysis Thermogravimetric (TGA) and Differential Scanning Calorimetry (DSC). The results obtained confirmed that the replacement of powdered avocado seed hydrolyzate (SPP) with sucrose in the HS medium for the production of CB by Komagataeibacter xylinus would not significantly affect or alter its properties.
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