Bibliographic citations
Díaz, K., Julca, M. (2021). Efecto de la melaza de caña de azúcar, glicerol y sulfato de amonio en la producción de biomasa, contenido de lípidos y cinética de crecimiento de Rhodosporidium toruloides [Universidad Nacional de Trujillo]. https://hdl.handle.net/20.500.14414/19659
Díaz, K., Julca, M. Efecto de la melaza de caña de azúcar, glicerol y sulfato de amonio en la producción de biomasa, contenido de lípidos y cinética de crecimiento de Rhodosporidium toruloides []. PE: Universidad Nacional de Trujillo; 2021. https://hdl.handle.net/20.500.14414/19659
@misc{renati/1045375,
title = "Efecto de la melaza de caña de azúcar, glicerol y sulfato de amonio en la producción de biomasa, contenido de lípidos y cinética de crecimiento de Rhodosporidium toruloides",
author = "Julca González, Manuel Alejandro",
publisher = "Universidad Nacional de Trujillo",
year = "2021"
}
ABSTRACT The use of oleaginous yeasts as an alternative source of oleochemical oil and biodiesel is of great interest, in this sense, the search for available substrates that provide high performance and low cost is important. The purpose of this study was to evaluate the effect of sugarcane molasses reducing sugars (MSR), glycerol (Gly) and ammonium sulfate (AS) on biomass production (ϕg), lipid content (L) and Rhodosporidium toruloides CCT 0783 NCYC growth kinetics. A Rotational Central Composite Design (RCDD) was used, performing 18 tests, with MRS, Gly and AS between 0.0 to 66.49 g/L, 0.0 and 50.0 g/L and 0.1 to 4.76 g/L respectively. The cultures were carried out in 250 mL flasks with a volume of 99 mL culture medium and inoculum of 1 mL at 250 rpm and a temperature of 28 ° C, for a time of 48 h. The highest content of L and ϕg was 52.37% and 4.487 g/L respectively with T11, using 33.24 g/L, and 2.43 g/L of MSR, and AS respectively, with a C/N ratio of 29.32. The use of the Gompertz model did not allow obtaining a statistical consistency of the experimental data (R2 between 0.76 to 0.93), due to the adaptation phase of R. toruloides cells to the culture medium and a high dispersion of growth kinetics.
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