Bibliographic citations
Portal, R., (2023). Susceptibilidad antimicrobiana de Escherichia coli aislada de pacientes con infección del tracto urinario atendidos en el Centro de Salud Contumazá – Cajamarca – 2021 [Universidad Nacional de Trujillo]. https://hdl.handle.net/20.500.14414/20720
Portal, R., Susceptibilidad antimicrobiana de Escherichia coli aislada de pacientes con infección del tracto urinario atendidos en el Centro de Salud Contumazá – Cajamarca – 2021 []. PE: Universidad Nacional de Trujillo; 2023. https://hdl.handle.net/20.500.14414/20720
@mastersthesis{renati/1044219,
title = "Susceptibilidad antimicrobiana de Escherichia coli aislada de pacientes con infección del tracto urinario atendidos en el Centro de Salud Contumazá – Cajamarca – 2021",
author = "Portal Pretel, Rosa Angélica",
publisher = "Universidad Nacional de Trujillo",
year = "2023"
}
n this investigation, the antimicrobial susceptibility of Escherichia coli isolated from patients with urinary tract infection treated at the Contumazá Health Center, Cajamarca, from January to July 2021, was determined. Urine samples were inoculated on Blood Agar (AS) and Agar Mac Conkey (AMC), then they were incubated at 37°C for 24 to 48 hours. Subsequently, colonies with macroscopic characteristics for E. coli were selected from the urine samples; with a count greater than 105 CFU/Ml on Mac Conkey Agar (AMC). E. coli was identified biochemically, through the following biochemical tests: urease, citrate, glucose, lactose and/or sucrose fermentation in TSI medium, lysine decarboxylation in LIA medium, hydrogen sulfide and indole production, mobility test in tubes with half a SIM and the VP-RM test. The isolated colonies identified as E. coli were inoculated on Trypticase Soy Agar (TSA) and incubated at 37°C for 24 h to obtain pure cultures. Then, the inoculum similar to tube No. 0.5 of the Mac Farland scale (1.5 x 108 CFU/mL) was prepared, which was seeded on Müeller Hinton agar plates. In order to establish antimicrobial susceptibility, using the plate diffusion disc method. For the evaluation, 10 antibiotics were used: Amikacin, Ceftazidime, Ceftriaxone, Amoxicillin/Ac. Clavulamic, Nitrofurantoin, Cephalexin, Gentamicin, Ciprofloxacin, Norfloxanin and Nalidixic Acid. The most outstanding results of antimicrobial susceptibility of E. coli were presented against Amikacin (90%), Ceftazidime (88%), Ceftriaxone (86%), Amoxicillin/Ac. Clavulamic (82%), Nitrofurantoin (80%), and Cephalexin (75%) and the highest resistance corresponded to Nalidixic acid (56%), Norfloxanine (54%), Ciprofloxacin (44%) and Gentamicin (36%).
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