Bibliographic citations
Rebaza, N., (2010). Capacidad biocida de Bacilllus thuringiensis SH-14 var. Israelensis y Beauveria bassiana sobre larvas de Aedes aegypti [Universidad Nacional de Trujillo]. https://hdl.handle.net/20.500.14414/19498
Rebaza, N., Capacidad biocida de Bacilllus thuringiensis SH-14 var. Israelensis y Beauveria bassiana sobre larvas de Aedes aegypti []. PE: Universidad Nacional de Trujillo; 2010. https://hdl.handle.net/20.500.14414/19498
@misc{renati/1044109,
title = "Capacidad biocida de Bacilllus thuringiensis SH-14 var. Israelensis y Beauveria bassiana sobre larvas de Aedes aegypti",
author = "Rebaza Sanchez, Neyde Lucelia",
publisher = "Universidad Nacional de Trujillo",
year = "2010"
}
In the present study evaluated the biocidal capacity of two entomopathogenic microorganisms: Bacillus thuringiensis SH-14 var. Israelensis (Bti SH-14) and Beauveria bassiana on the third stage larvae of Aedes aegypti in laboratory conditions and also determined the median lethal dose (DL50). Strains H-14 Bacillus thuringiensis var. Israelensis (Bti SH-14) and Beauveria bassiana were provided by the Laboratory of Cellular and Molecular Biology, School of Biological Sciences, National University of Trujillo. The larvae of A. aegypti from which standardized the cycle were collected Laredo district, province of Trujillo, Department of Libertad. Una having obtained the eggs were kept in trays with distilled water to hatch, the larvae were retained in the laboratory at room temperature between 26 and 28 º C and a relative humidity between 80 and 82%. Bioassays were conducted with 12 experimental groups and 2 control groups with three replicates of 25 larvae in 100 mL of distilled water each. 6 experimental groups were used with B. thuringiensis SH-14 var. Israelensis, the concentrations of 670esp/mL; 1683esp/mL; 4227esp/mL; 10619esp/mL; 26673esp/mL and 67000esp/mL, and their respective control group, yielding a mortality of 50% at 8 hours and 100% at 24 hours. And with B. Bassiana were used the other 6 experimental groups at concentrations of 4 x 108esp/mL, 1 x 109esp/mL; 2.5 x 109esp/ml; 6.3 x 109esp/mL; 1.6 x 1010esp / ml and 4 x 1010esp/mL, and respect control group, yielding a 50% mortality at 7 days and 100% at 9 days. Was determined by median lethal dose (DL50) both in B. thuringiensis SH-14 var. Israelensis as Beauveria bassiana, a higher concentration of spores will be increased mortality of larvae in less time. We conclude that Bacillus thuringiensis SH – 14 var. Israelensis and B.bassiana to the different concentrations used in bioassays, have a high 3 biocidal capacity on the third stage larvae of A. aegypti in laboratory conditions.
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