Bibliographic citations
Lavado, D., (2024). Determinación de la actividad antimicrobiana in vitro de las trampas extracelulares de neutrófilos purificados de pacientes con tuberculosis [Universidad Nacional de Trujillo]. https://hdl.handle.net/20.500.14414/22622
Lavado, D., Determinación de la actividad antimicrobiana in vitro de las trampas extracelulares de neutrófilos purificados de pacientes con tuberculosis []. PE: Universidad Nacional de Trujillo; 2024. https://hdl.handle.net/20.500.14414/22622
@mastersthesis{renati/1043842,
title = "Determinación de la actividad antimicrobiana in vitro de las trampas extracelulares de neutrófilos purificados de pacientes con tuberculosis",
author = "Lavado Vega, Danyer Ericksen",
publisher = "Universidad Nacional de Trujillo",
year = "2024"
}
Objective: To determine the in vitro antimicrobial activity of extracellular traps of purified neutrophils from patients with tuberculosis. Method: This work is based on the use of the MODS methodology, which allows the detection of M. tuberculosis and allows the evaluation of susceptibility to antimicrobials. The NETs will be obtained by purification of sputum samples from patients with tuberculosis, by centrifugation. Four dilutions of purified NETs will be used (25%, 50%, 75%, 90%). The efficacy evaluation of the purified NETs extract against Mycobacterium tuberculosis was carried out in a 24-well flat bottom sterile polystyrene plate which contains the different dilutions of NETs at which the bacteria were subsequently inoculated. Then, the observations obtained from the control wells were contrasted against the wells that contained the different concentrations of NETs. Results: No antimicrobial effect was observed in any of the concentrations. That is, all concentrations (25, 50, 75, 90%) yielded positive results, not finding significant differences with the control group (Mycobacterium tuberculosis wells without NETs). Conclusion: Purified neutrophil extracellular traps (NETs) have no antimicrobial efficacy against Mycobacterium tuberculosis strains.
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