Alcántara, L. (2022). Eficacia de control de Bacillus thuringiensis var. Kurstaki sobre Spodoptera frugiperda en espárrago y su efecto residual en invernadero. [, Universidad Nacional de Trujillo]. http://dspace.unitru.edu.pe/handle/UNITRU/19481
Alcántara, L. Eficacia de control de Bacillus thuringiensis var. Kurstaki sobre Spodoptera frugiperda en espárrago y su efecto residual en invernadero. . PE: Universidad Nacional de Trujillo; 2022. http://dspace.unitru.edu.pe/handle/UNITRU/19481
title = "Eficacia de control de Bacillus thuringiensis var. Kurstaki sobre Spodoptera frugiperda en espárrago y su efecto residual en invernadero.",
author = "Alcántara Espino, Luisa Micaela",
publisher = "Universidad Nacional de Trujillo",
year = "2022"
S. frugiperda is considered a key pest in asparagus causing feeding damage to both branches and twigs and has even been found to damage freshly harvested green asparagus shoots. As part of phytosanitary management, chemical products are rotated in addition to biological products, thus we have B. thuringiensis var. Kurstaki, used both in crop maintenance and at harvest time. The present investigation aimed to evaluate the control efficacy of three strains of B. thuringiensis var. Kurstaki in different doses: BTK 32 WP (Strain W-K) at 400, 500 and 600 g / 200 L; BTK 50 WP (Strain W-K) at 400, 500 and 600 g / 200 L; faced with a commercial product of B.thuringiensis var. Kurstaki (Strain S-L) at a dose of 500g / 200 L (commercial dose) and an absolute control, under field conditions. As a result, it was obtained that BTK 32 WP (Strain W-K) at 500 g / 200 L presented a control of 98% at 12 days after inoculation, with no statistically significant difference between the treatments and the established dose. After knowing the best product in the asparagus field, we proceeded to evaluate and determine the Residual Effect, for this, a breeding of S. frugiperda was carried out in the laboratory and sowing of corn in a greenhouse, which was inoculated BTK 32 WP (Strain WK) at doses of 500 g / 200 L, and in the following days, larvae of the 2nd and 3rd stage were fed with corn leaves, grouping a total of 33 larvae (divided into 3 repetitions) by date after BTK inoculation. that we had groups at 3, 6, 9, and 12 days after the corn husks were exposed to solar radiation, and then fed them. When finding dead larvae in each group, they were subjected to heat treatment to determine the presence of B. thuringiensis. The residual effect expressed in larval mortality (%) of S. frugiperda, managed to show that the parasporal crystal is affected by solar radiation after having kept the corn plants exposed to environmental conditions for 12 days, observing at 3-, 6-, 9- and 12-days larval mortality of 67%, 21%, 12% and 3%, respectively. Likewise, the evaluation of the Residual Effect, allowed to verify the presence of the parasporal crystal in dead larvae of each feeding date.
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