Asunción, H. (2021). Implementación de un protocolo para evaluar la reactividad contráctil ex-vivo del íleon de rata en baño de tejido aislado [, Universidad Nacional de Trujillo]. http://dspace.unitru.edu.pe/handle/UNITRU/16337
Asunción, H. Implementación de un protocolo para evaluar la reactividad contráctil ex-vivo del íleon de rata en baño de tejido aislado . PE: Universidad Nacional de Trujillo; 2021. http://dspace.unitru.edu.pe/handle/UNITRU/16337
title = "Implementación de un protocolo para evaluar la reactividad contráctil ex-vivo del íleon de rata en baño de tejido aislado",
author = "Asunción Alvarez, Holbert Daniel",
publisher = "Universidad Nacional de Trujillo",
year = "2021"
The isolated tissue bath system has been used by pharmacology for many years for the characterization of receptors and their signal transduction. In particular, the isolated ileum is one of the most widely used models in this field of research. The objective of this study was the implementation of an experimental protocol to evaluate the ex-vivo contractile reactivity of rat ileum in an isolated tissue bath. The protocol begins by detailing the steps to assemble the isolated tissue bath, as well as its calibration, since this is a crucial stage in order to obtain reliable research data. The ileal segments were obtained from male Rattus norvegicus Holtzman (n = 9) and a PowerLab 26T system was used to record contractile reactivity. The protocol began by evaluating the effect of an unknown substance on the basal tone. Second, it was demonstrated that using pre-contracted segments with acetylcholine (10-5 M ACh), serotonin (10-5 M 5-HT) and 80 mM BaCl2, the spasmolytic effect (therapeutic effect) of a substance could be studied. Likewise, to understand the antispasmodic effect (preventive effect), dose-response curves were constructed against ACh (10-10 M-10-4 M) in order to know if the sensitivity (pEC50) of the tissue is modified or not by means of the addition of an exogenous substance. Then, to evaluate the role of the influx of extracellular Ca2+ in the contractile response to ACh, a protocol was developed using a Ca2+ -free Tyrode solution. Finally, to study whether the antispasmodic effects of the tested substances were associated with their antioxidant activity, the ileal segments were pre-incubated for 20 min with the sample, before contraction with hydrogen peroxide (H2O2) as pro-oxidant substance.
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