Bibliographic citations
Flores, J., (2004). Actividad de la uridín difosfoglucosa deshidrogenasa (E.C. 1.1.1.22) en hígado de rata tratada crónicamente con cocaína [Tesis, Universidad Nacional Mayor de San Marcos]. https://hdl.handle.net/20.500.12672/1104
Flores, J., Actividad de la uridín difosfoglucosa deshidrogenasa (E.C. 1.1.1.22) en hígado de rata tratada crónicamente con cocaína [Tesis]. PE: Universidad Nacional Mayor de San Marcos; 2004. https://hdl.handle.net/20.500.12672/1104
@misc{sunedu/2871308,
title = "Actividad de la uridín difosfoglucosa deshidrogenasa (E.C. 1.1.1.22) en hígado de rata tratada crónicamente con cocaína",
author = "Flores Benavides, Javier Richard",
publisher = "Universidad Nacional Mayor de San Marcos",
year = "2004"
}
In the present investigation work the activity of the enzyme UDPG dehydrogenase was determined in chronically cocaine-treated rats utilizing the oral route. To approach this three groups of rats were prepared, 1 control and 2 experimental, all three consuming different casein 9%-based diets. The diets of the 2 experimental groups C7.5 and C15, unlike of the control group that only contained casein, had different cocaine concentrations of 7.5 mg/10g diet and 15 mg/10g diet respectively. All diets were fed ad libitum for a period of 22 days to the three groups (n=7). Ending this period the animals were decapitated and the liver was removed, from which, the supernatant 12000 g fraction and the cytosolic fraction were obtained through differential centrifugation. These two subcellular fractions were used to measure the UDPG dehydrogenase activity, as well as their respective protein content. The results showed increased UDPG dehydrogenase activity in the experimental groups, which is directly proportional to the amount of cocaine consumed. This increasing in activity occurred in both subcellular fractions. The values of micromoles of UDPGA/mg protein formed in the supernatant 12000 g fraction were: Control, 5.49x10ˉ³ ± 5.69x10ˉ³; C7.5, 7.67x10ˉ³ ± 2.94x10ˉ³; and C15, 10.60 x10ˉ³ ± 3.35x10ˉ³. In the cytosol the values were: Control, 12.20 x10ˉ³ ± 4.13x10ˉ³; C7.5, 13.10 x10ˉ³ ± 3.77x10ˉ³; and C15, 14.80 x10ˉ³ ± 3.93x10ˉ³. Moreover, a major protein content in both subcellular fractions of the experimental groups was obtained and also a lowering in the body weight by the action of cocaine. These findings suggest that the increased UDPG dehydrogenase activity would be due to the induction of this enzyme synthesis, therefore producing UDPGA, which would be used for the conjugation and excretion of the cocaine metabolites resulting from its biotransformation in the body.
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